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“Dietary supplementation of L-tryptophan” increases muscle development, adipose tissue catabolism and fatty acid transportation in the muscles of Hanwoo steers

한국축산학회지 2020년 62권 5호 p.595 ~ 604
Priatno Wahyu, 조용호, Nejad Jalil Ghassemi, 이재성, Moon Jun-Ok, 이홍구,
소속 상세정보
 ( Priatno Wahyu ) - Konkuk University Sanghuh College of Life Sciences Department of Animal Science and Technology
조용호 ( Jo Yong-Ho ) - Konkuk University Sanghuh College of Life Sciences Department of Animal Science and Technology
 ( Nejad Jalil Ghassemi ) - Konkuk University Sanghuh College of Life Sciences Department of Animal Science and Technology
이재성 ( Lee Jae-Sung ) - Konkuk University Sanghuh College of Life Sciences Department of Animal Science and Technology
 ( Moon Jun-Ok ) - CJ CheilJedang Institute of Biotechnology
이홍구 ( Lee Hong-Gu ) - Konkuk University Sanghuh College of Life Sciences Department of Animal Science and Technology

Abstract


This study investigated the effects of dietary rumen-protected L-tryptophan (TRP) supplementation (43.4 mg of L-tryptophan kg?1 body weigt [BW]) for 65 days in Hanwoo steers on muscle development related to gene expressions and adipose tissue catabolism and fatty acid transportation in longissimus dorsi muscles. Eight Hanwoo steers (initial BW = 424.6 kg [SD 42.3]; 477 days old [SD 4.8]) were randomly allocated to two groups (n = 4) of control and treatment and were supplied with total mixed ration (TMR). The treatment group was fed with 15 g of rumen-protected TRP (0.1% of TMR as-fed basis equal to 43.4 mg of TRP kg?1 BW) once a day at 0800 h as top-dressed to TMR. Blood samples were collected 3 times, at 0, 5, and 10 weeks of the experiment, for assessment of hematological and biochemical parameters. For gene study, the longissimus dorsi muscle samples (12 to 13 ribs, approximately 2 g) were collected from each individual by biopsy at end of the study (10 weeks). Growth performance parameters including final BW, average daily gain, and gain to feed ratio, were not different (p > 0.05) between the two groups. Hematological parameters including granulocyte, lymphocyte, monocyte, platelet, red blood cell, hematocrit, and white blood cell showed no difference (p > 0.05) between the two groups except for hemoglobin (p = 0.025), which was higher in the treatment than in the control group. Serum biochemical parameters including total protein, albumin, globulin, blood urea nitrogen, creatinine phosphokinase, glucose, nonesterified fatty acids, and triglyceride also showed no differences between the two groups (p > 0.05). Gene expression related to muscle development (Myogenic factor 6 [MYF6], myogenine [MyoG]), adipose tissue catabolism (lipoprotein lipase [LPL]), and fatty acid transformation indicator (fatty acid binding protein 4 [FABP4]) were increased in the treatment group compared to the control group (p < 0.05). Collectively, supplementation of TRP (65 days in this study) promotes muscle development and increases the ability of the animals to catabolize and transport fat in muscles due to an increase in expressions of MYF6, MyoG, FABP4, and LPL gene.

키워드

Adipose tissue catabolism; Fatty acid transportation; Growth performance; Hanwoo steers; Muscle development; Rumen-protected L-tryptophan

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