잠시만 기다려 주세요. 로딩중입니다.

Exploring the feasibility of Salmonella Typhimurium-specific phage as a novel bio-receptor

한국축산학회지 2020년 62권 5호 p.668 ~ 681
최인영, 박도현, Chin Brayan A., 이정훈, 이진영, 박미경,
소속 상세정보
최인영 ( Choi In-Young ) - Kyungpook National University School of Food Science and Biotechnology
박도현 ( Park Do-Hyeon ) - Kyungpook National University School of Food Science and Biotechnology
 ( Chin Brayan A. ) - Auburn University Materials Research and Education Center
이정훈 ( Lee Cheong-Hoon ) - Seoul National University Graduate School of Public Health
이진영 ( Lee Jin-Young ) - Sangmyung University Gyedong General Education Institute
박미경 ( Park Mi-Kyung ) - Kyungpook National University School of Food Science and Biotechnology

Abstract


The purpose of this study was aimed to isolate a Salmonella Typhimurium-specific phage (KFS-ST) from washing water in a poultry processing facility and to investigate the feasibility of the KFS-ST as a novel bio-receptor for the magnetoelastic (ME) biosensor method. KFS-ST against S. Typhimurium was isolated, propagated, and purified using a CsCl-gradient ultracentrifugation. Morphological characteristics of KFS-ST were analyzed using transmission electron microscopy (TEM). Its specificity and efficiency of plating analysis were conducted against 39 foodborne pathogens. The temperature and pH stabilities of KFS-ST were investigated by the exposure of the phage to various temperatures (?70°C?70°C) and pHs (1?12) for 1 h. A one-step growth curve analysis was performed to determine the eclipse time, latent time and burst size of phage. The storage stability of KFS-ST was studied by exposing KFS-ST to various storage temperatures (?70°C, ?20°C, 4°C, and 22°C) for 12 weeks. KFS-ST was isolated and purified with a high concentration of (11.47 ± 0.25) Log PFU/mL. It had an icosahedral head (56.91 ± 2.90 nm) and a non-contractile tail (225.49 ± 2.67 nm), which was classified into the family of Siphoviridae in the order of Caudovirales. KFS-ST exhibited an excellent specificity against only S. Typhimurium and S. Enteritidis, which are considered two of the most problematic Salmonella strains in the meat and poultry. However, KFS-ST did not exhibit any specificity against six other Salmonella and 27 non-Salmonella strains. KFS-ST was stable at temperature of 4°C to 50°C and at pH of 4 to 12. The eclipse time, latent time, and burst size of KFS-ST were determined to be 10 min, 25 min and 26 PFU/ infected cell, respectively. KFS-ST was relatively stable during the 12-week storage period at all tested temperatures. Therefore, this study demonstrated the feasibility of KFS-ST as a novel bio-receptor for the detection of S. Typhimurium and S. Enteritidis in meat and poultry products using the ME biosensor method.

키워드

Salmonella-specific phage; Bio-receptor; Magnetoelastic biosensor; Meat and poultry

원문 및 링크아웃 정보

등재저널 정보