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Inhibitory effects of Porphyra dentata extract on 3T3-L1 adipocyte differentiation

한국축산학회지 2020년 62권 6호 p.854 ~ 863
최수영, 이수연, 장다혜, 이석준, 조정용, 김성학,
소속 상세정보
최수영 ( Choi Su-Young ) - Chonnam National University Department of Animal Science
이수연 ( Lee Su-Yeon ) - Chonnam National University Department of Food Science and Technology
장다혜 ( Jang Da-Hye ) - Chonnam National University Department of Food Science and Technology
이석준 ( Lee Suk-Jun ) - Cheongju University College of Health Science Department of Biomedical Laboratory Science
조정용 ( Cho Jeong-Yong ) - Chonnam National University Department of Food Science and Technology
김성학 ( Kim Sung-Hak ) - Chonnam National University Department of Animal Science

Abstract


This study was aimed to investigate the inhibitory effects of Porphyra dentata (P. dentata) extract on the adipogenesis of 3T3-L1 cells and evaluate its anti-obesity effect. The proliferation of 3T3-L1 cells and differentiation of adipocytes under treatment of P. dentata extract was examined by measuring the cell viability using alamarBlue assay and lipid droplets by Oil Red O staining. Results showed that P. dentata extract has no cytotoxicity effect and lipid droplets formation decreased in a concentration-dependent manner in 3T3-L1 cells. It has been confirmed that transcription factors affecting lipid accumulation and anti-adipogenic effects during cell differentiation are linked to P. dentata extract. We observed that P. dentata shows lowering the mRNA expression of peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα) that adipogenesis-associated key transcription factors and inhibiting adipogenesis in the early stages of differentiation. Treating the cells with P. dentata did not only suppressed PPARγ2 and C/EBPα but also significantly decreased the mRNA expression of adiponectin, Leptin, fatty acid synthase, adipocyte protein 2, and Acetyl-coA carboxylase 1. Overall, the P. dentata extract demonstrated inhibitory property in adipogenesis, which has a potential effect in anti-obesity in 3T3-L1 cells.

키워드

3T3-L1; Adipocyte differentiation; Adipogenesis; Porphyra dentata; Anti-obesity

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