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Reanalysis of discarded blastocysts for autosomal aneuploidy after sex selection in cleavage-stage embryos

Clinical and Experimental Reproductive Medicine 2020년 47권 4호 p.293 ~ 299
Ebrahimian Neda, Montazeri Fatemeh, Sadeghi Mohammad Reza, Kalantar Seyed Mehdi, Gilany Kambiz, Khalili Mohannad Ali,
소속 상세정보
 ( Ebrahimian Neda ) - Shahid Sadoughi University of Medical Sciences Yazd Reproductive Sciences Institute Research and Clinical Center for Infertility
 ( Montazeri Fatemeh ) - Shahid Sadoughi University of Medical Sciences Yazd Reproductive Sciences Institute Abortion Research Center
 ( Sadeghi Mohammad Reza ) - Academic Center for Education, Culture and Research Avicenna Research Institute Reproductive Embryology and Andrology Research Center
 ( Kalantar Seyed Mehdi ) - Shahid Sadoughi University of Medical Sciences Yazd Reproductive Sciences Institute Abortion Research Center
 ( Gilany Kambiz ) - Academic Center for Education, Culture and Research Avicenna Research Institute Reproductive Biotechnology Research Center
 ( Khalili Mohannad Ali ) - Shahid Sadoughi University of Medical Sciences Yazd Reproductive Sciences Institute Research and Clinical Center for Infertility

Abstract


Objective: The goal of the present study was to investigate the rate of chromosomal aneuploidies in surplus embryos after sex determination at the cleavage stage. Then, the same chromosomal aneuploidies were evaluated in blastocysts after extended culture.

Methods: Sixty-eight surplus embryos were biopsied at the cleavage stage and incubated for an additional 3 days to allow them to reach the blastocyst stage. The embryos were reanalyzed via fluorescence in situ hybridization (FISH) to examine eight chromosomes (13, 15, 16, 18, 21, 22, X, and Y) in both cleavage-stage embryos and blastocysts.

Results: Although the total abnormality rate was lower in blastocysts (32.35%) than in cleavage-stage embryos (45.58%), the difference was not significant (p=0.113). However, when we restricted the analysis to autosomal abnormalities, we observed a significant difference in the abnormality rate between the cleavage-stage embryos (44.11%) and the blastocysts (17.64%, p=0.008). A higher rate of sex chromosomal abnormalities was also observed in cleavage-stage embryos (29.4%) than in blastocysts (14.70%, p=0.038).

Conclusion: The data indicated that embryo biopsy should be conducted at the blastocyst stage rather than the cleavage stage. The results also emphasized that examination of common chromosomal aneuploidies apart from sex selection cycles can be conducted in the blastocyst stage with the FISH method.

키워드

Blastocyst; Embryo; Fluorescence in situ hybridization; Trophectoderm biopsy

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