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Methylparaben induces cell-cycle arrest and caspase-3-mediated apoptosis in human placental BeWo cells

Molecular & Cellular Toxicology 2020년 16권 4호 p.409 ~ 418
김지영, 김미진, Shin Geun-Seup, 이현민, 김철홍, 김정웅,
소속 상세정보
김지영 ( Kim Ji-Young ) - Chung-Ang University Department of Life Science
김미진 ( Kim Mi-Jin ) - Chung-Ang University Department of Life Science
 ( Shin Geun-Seup ) - Chung-Ang University Department of Life Science
이현민 ( Lee Hyun-Min ) - Chung-Ang University Department of Life Science
김철홍 ( Kim Chul-Hong ) - Chung-Ang University Department of Life Science
김정웅 ( Kim Jung-Woong ) - Chung-Ang University Department of Life Science

Abstract


Background: Parabens are frequently used as antimicrobial preservatives in various commercials, pharmaceutical products, and foods. Recent studies have suggested that the exposure of pregnant women to methylparaben has adverse effects on the fetus. However, the biological mechanisms underlying the effects of methylparaben on human placental cells are yet to be elucidated.

Objective: In this study, we determined that methylparaben induces cell death by affecting the growth, cell-cycle arrest, and apoptosis of human placental BeWo cells. We employed flow cytometry at the single-cell level for analyzing the expression of proteins related to cell cycle and apoptosis instead of using conventional methods.

Results: The results demonstrated that treatment with methylparaben significantly decreased cell viability. We observed that methylparaben increased the protein level of cyclin B1, which arrested the cell cycle at sub-G1 status, and decreased the proportion of BeWo cells in the G2/M phase. We also observed that methylparaben induced caspase-3-mediated apoptosis in a time- and dose-dependent manner.

Conclusion: These results suggested that methylparaben had cytotoxic adverse effects on human BeWo placenta cells through the induction of apoptosis and cell-cycle arrest.

키워드

Apoptosis; Methylparaben; Caspase-3; Human placenta; BeWo cells

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