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Curcumin promotes oxidative stress, apoptosis and autophagy in H9c2 rat cardiomyoblasts

Molecular & Cellular Toxicology 2020년 16권 4호 p.441 ~ 453
Zepeda-Quiroz Ivan, Sanchez-Barrera Helen, Colin-Val Zaira, Robledo-Cadena Diana Xochiquetzal, Rodriguez-Enriquez Sara, Lopez-Marure Rebeca,
소속 상세정보
 ( Zepeda-Quiroz Ivan ) - Instituto Nacional de Cardiologia Ignacio Chavez Departamento de Fisiologia
 ( Sanchez-Barrera Helen ) - Instituto Nacional de Cardiologia Ignacio Chavez Departamento de Fisiologia
 ( Colin-Val Zaira ) - Instituto Nacional de Cardiologia Ignacio Chavez Departamento de Fisiologia
 ( Robledo-Cadena Diana Xochiquetzal ) - Instituto Nacional de Cardiologia Ignacio Chavez Departamento de Bioquimica
 ( Rodriguez-Enriquez Sara ) - Instituto Nacional de Cardiologia Ignacio Chavez Departamento de Bioquimica
 ( Lopez-Marure Rebeca ) - Instituto Nacional de Cardiologia Ignacio Chavez Departamento de Fisiologia

Abstract


Background: Curcumin, a polyphenol derived from Curcuma longa, has some adverse effects on heart; however, its toxic effects on cardiac cells are poorly understood.

Objective: To evaluate the toxicity of curcumin on H9c2 rat cardiomyoblasts. To this, H9c2 cells were exposed to different concentrations of curcumin and proliferation, viability, cell cycle, oxidative stress, mitochondrial membrane potential (ΔΨm), death and autophagy were evaluated.

Results: Curcumin caused concentration-dependent inhibition of H9c2 cells proliferation and viability. A higher sub-G1 population was observed in cells treated with curcumin, which was related with phosphatidylserine translocation and increase of activated caspase-9, indicating apoptotic death. Curcumin induced oxidative stress and decreased ΔΨm causing mitochondrial dysfunction. Additionally, it promoted autophagy, revealed by higher LC3B and beclin-1 protein expression and mitophagy.

Conclusion: Curcumin exhibited toxic effects in cardiac cells and further studies are required to validate its therapeutic potential and use as anti-inflammatory and anti-oxidant agent in the cardiovascular system.

키워드

Curcumin; Cardiomyoblasts; Cell cycle; Oxidative stress; Cell death; Mitochondrial damage; Autophagy; Mitophagy

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