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Decreased plasma α-synuclein in idiopathic Parkinson’s disease patients after adjusting hemolysis factor

Molecular & Cellular Toxicology 2020년 16권 4호 p.477 ~ 484
심규환, 김승찬, 윤영철, 성영희, 안성수,
소속 상세정보
심규환 ( Shim Kyu-Hwan ) - Veterans Health Service Medical Center Department of Neurology
김승찬 ( Kim Seung-Chan ) - Gachon University Department of BioNano Technology
윤영철 ( Youn Young-Chul ) - Chung-Ang University College of Medicine Department of Neurology
성영희 ( Sung Young-Hee ) - Gachon University Gil Medical Center Department of Neurology
안성수 ( An Seong-Soo A. ) - Gachon University Department of BioNano Technology

Abstract


Background: Lewy bodies are pathological hallmarks for the diagnosis of Parkinson’s disease, where the core components are composed of aggregated forms of α-synuclein (α-syn). Although α-syn has been investigated as a potential biomarker for PD, its usage has been limited and still remains controversial.

Objective: An accurate enzyme-linked immunosorbent assay (ELISA) was developed for the detection of α-syn in plasma. The hemolysis score was calculated to eliminate the additive α-syn levels from RBCs. Human plasma samples were collected in heparinized blood tubes from idiopathic Parkinson disease (IPD) and healthy control (HC).

Result: Hemolysis score had a strong correlation with the level of plasma α-syn. From the limited set of samples in this preliminary study, decreased α-syn concentrations were observed in patients with IPD in comparison to HC after adjusting for hemolysis factor. Similar results with a commercial ELISA kit were found for measuring α-syn from the same set of samples with lower correlation and the reduced accuracy of diagnosis than the current study.

Conclusion: The adjustments for the hemolysis factor would be indispensable, supporting plasma α-syn as a potential surrogate biomarker for distinguishing IPD from HC.

키워드

Parkinson’s disease; α-Synuclein; Hemolysis

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