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RBL-2H3 cell에서 며느리배꼽 열수추출물의 알러지 억제 효과

Inhibitory effect of Persicaria perfoliata (L.) H. Gross on IgE mediated allergic responses in RBL-2H3 cells

대한통합의학회지 2020년 8권 4호 p.163 ~ 169
윤현서, 박충무,
소속 상세정보
윤현서 ( Yoon Hyun-Seo ) - Dong-Eui University Department of Dental Hygiene
박충무 ( Park Chung-Mu ) - Dong-Eui University Department of Clinical Laboratory Science

Abstract


Purpose : : This study aimed to investigate the anti-allergic effect of Persicaria perfoliata water extract (PPWE) on IgE stimulated rat basophilic leukemia (RBL-2H3) cell line.

Method : : P. perfoliata (L.) H. Gross has been used in traditional medicine as an anti-allergic agent, antipyretic, and diuretic and for respiratory disorders. To analyze the anti-allergic activity of PPWE, release of β-hexosaminidase in RBL-2H3 cells was estimated by enzyme linked immunosorbant assay (ELISA). Also, the cytotoxic effect of PPWE was identified by WST assay, and nuclear factor (NF)-κB and its upstream signaling molecules were assessed by western blot analysis.

Result : : PPWE treatment significantly attenuated β-hexosaminidase release in a dose dependent manner without any cytotoxicity. PPWE inhibited β-hexosaminidase activity by 38.4 ± 1.2, 36.58 ± 0.6, 32.5 ± 2.2, and 26.5 ± 1.2 at 500, 250, 100, and 50 μg/ml of PPWE, respectively, compared with the control group. In addition, an analysis of the expression level of NF-κB, an inflammatory transcription factor, in RBL-2H3 cells upon IgE stimulation provided results consistent with the results of β-hexosaminidase release. The phosphorylated status of upstream signaling molecules for transcription factor, mitogen activated protein kinases (MAPKs), was also analyzed. The results showed that PPWE treatment dose-dependently inhibited phosphorylation of extracellular regulatory kinase (ERK) and c-Jun N-terminal kinase (JNK). These results show that PPWE had a strong IgE-mediated degranulation inhibitory effect on RBL-2H3 cells

Conclusion : : P. perfoliata ameliorated IgE-mediated allergic reaction via the modulation of MAPK and NF-κB signaling pathway in RBL-2H3 cells. These results indicate that P. perfoliata could be a potential candidate for a treatment strategy against various allergic disorders.

키워드

Antiallergic effect; Mitogen activated protein kinase; Nuclear factor-κB; Persicaria perfoliata (L.) H. Gross; RBL-2H3

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