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성숙도에 따른 복분자 열매의 미백 활성 비교

Comparison of whitening effect of Rubus coreanus fruit according to maturity

Journal of Nutrition and Health 2020년 53권 2호 p.121 ~ 128
박정용, 이지연, 서경혜, 장귀영, 이승은, 지윤정, 김형돈,
소속 상세정보
박정용 ( Park Jeong-Yong ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research
이지연 ( Lee Ji-Yeon ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research
서경혜 ( Seo Kyung-Hye ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research
장귀영 ( Jang Gwi-Young ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research
이승은 ( Lee Seung-Eun ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research
지윤정 ( Ji Yun-Jeong ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research
김형돈 ( Kim Hyung-Don ) - National Instiute of Horticultural & Herbal Science Department of Herbal Crop Research

Abstract


Purpose: The Rubus coreanus fruit (RF) is an important traditional medicinal herb having antioxidant, anti-inflammatory, and immunoregulatory properties. These activities are known to change dramatically, depending on maturity of the RF. It is presumed that change of functional components, such as flavonoids, tannins, phenolic acids, triterpenoids and organic acids in RF, affect the various bioactivities. This study aimed to confirm changes in the anti-melanogenic effects of RF based on maturity, and to identify the bioactive compounds responsible.

Methods: The cell viability of mature RF (MRF) and immature RF (IRF) extracts was investigated using B16F10 cells. To compare the anti-melanogenic effect of MRF and IRF extracts, we first assessed the melanin content. High-performance liquid chromatography analysis was performed to evaluate changes in the level of ellagic acid according to maturity of the RF. In addition, tyrosinase inhibitory activity of both extracts was examined.

Results: MRF and IRF extracts (50?200 μg/mL) do not affect the cell viability of B16F10 melanoma cells. IRF extract more effectively inhibited melanin synthesis than MRF extract. The content of ellagic acid in IRF extract was higher than that obtained in MRF extract. Furthermore, greater inhibition of tyrosinase activity was observed after exposure to IRF extract than MRF extract. A positive correlation was determined between ellagic acid content and tyrosinase inhibitory activity, and a negative correlation was obtained between ellagic acid content and melanin content. Taken together, our results indicate that ellagic acid is one of the major bioactive compounds of RF that imparts a whitening effect.

Conclusion: Our results indicate that ellagic acid in MRF and IRF extracts affect the anti-melanogenesis effect through inhibition of tyrosinase activity. Therefore, the ellagic acid rich IRF has greater potential for application as a natural and functional cosmetic material.

키워드

Rubus coreanus; ellagic acid; melanoma cell; tyrosinase; melanin

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