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Design and Evaluation of a Scalding Animal Model by the Boiling Water Method

Hua Cheng, Lyu Lele, Ryu Hyun-Seok, 박소영, 임남규, Abueva Celine D. G., 정필상,
소속 상세정보
 ( Hua Cheng ) - Dankook University Beckman Laser Institute Korea
 ( Lyu Lele ) - Dankook University Beckman Laser Institute Korea
 ( Ryu Hyun-Seok ) - Dankook University Beckman Laser Institute Korea
박소영 ( Park So-Young ) - Dankook University Beckman Laser Institute Korea
임남규 ( Lim Nam-Kyu ) - Dankook University Hospital Department of Plastic and Reconstructive Surgery
 ( Abueva Celine D. G. ) - Dankook University Beckman Laser Institute Korea
정필상 ( Chung Phil-Sang ) - Dankook University Beckman Laser Institute Korea

Abstract


Background and Objectives: For experiments on simulated burn, the preparation of an animal model is a very important step. The purpose of the current experiment is to design a simple and controllable method for the preparation of third-degree scald in a mouse model using the boiling water method.

Materials and Methods: A total of 18 Swiss mice were used. After the anesthetization, the mice were scalded by boiling water (100°C) using a mold with a 1 cm2 circle area on the dorsum at contact times of 3s, 5s, and 8s. After confirming that 8 seconds of scald can cause a third-degree scald, the skin samples were collected at day 2, 4, 6, 8, 10, and 12, and analyzed by histopathological examinations. The wound retraction index (WRI) was also measured.

Results: Third-degree scald involving full-thickness skin was observed in the 8-second scald group, while a 3-second scald caused a superficial second-degree scald and a 5-second scald caused a deep second-degree scald. After third-degree scald, the burn wound continued to contract until day 14.

Conclusion: The scalding model of mice can be successfully established by the boiling water method. This method is easy to operate, it has a low cost, and it can control the scald depth by controlling the scald time. This is adequate to study skin thermal injury in the future. The scald model established by this method can last for 14 days.

키워드

Mouse model; Burn; Scald

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