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IGF2 and IGF1R mRNAs Are Detectable in Human Spermatozoa

The World Journal of Men′s Health 2020년 38권 4호 p.545 ~ 551
Cannarella Rossella, Condorelli Rosita A., La Vignera Sandro, Bellucci Catia, Calafiore Riccardo, Calogero Aldo E.,
소속 상세정보
 ( Cannarella Rossella ) - University of Catania Department of Clinical and Experimental Medicine
 ( Condorelli Rosita A. ) - University of Catania Department of Clinical and Experimental Medicine
 ( La Vignera Sandro ) - University of Catania Department of Clinical and Experimental Medicine
 ( Bellucci Catia ) - University of Perugia Department of Experimental Medicine
 ( Calafiore Riccardo ) - University of Perugia Department of Experimental Medicine
 ( Calogero Aldo E. ) - University of Catania Department of Clinical and Experimental Medicine

Abstract


Purpose: Oligozoospermia is highly prevalent worldwide. Studies have reported a lower methylation rate in the H19 differentially methylated region at the sperm level in oligozoospermic patients than in controls. IGF2/H19 are the best-known pair of imprinted genes. However, no studies have yet evaluated whether they are transcribed in human sperm. To assess whether IGF2 and IGF1R mRNAs are present in human sperm and if their levels are correlated with sperm concentration and total sperm count.

Materials and Methods: Sperm samples (n=22) underwent reverse-transcription quantitative polymerase chain reaction using specific primers to detect IGF2 and IGF1R mRNA levels. They were then correlated with patients' conventional sperm parameters using the Spearman (τ) and Kendall (ρ) rank correlation coefficients.

Results: Statistically significant positive correlations were found between IGF2 mRNA levels and sperm concentration (τ=0.403, p<0.01; ρ=0.587, p<0.005) and total sperm count (τ=0.347, p<0.024; ρ=0.509, p<0.015). IGF1R mRNA levels were positively correlated with sperm concentration (τ=0.595, p<0.001; ρ=0.774, p<0.001) and total sperm count (τ=0.547, p<0.001; ρ=0.701, p<0.001). Apart from IGF1R mRNA and sperm morphology (τ=0.325, p<0.05; ρ=0.461, p<0.05), no additional correlations were found between the levels of these transcripts and other conventional sperm parameters.

Conclusions: IGF2 and IGF1R mRNAs were found to be present in human spermatozoa and their transcription levels were positively correlated with sperm concentration and total sperm count. Spermatozoa are the only source of IGF2 mRNA since IGF2 is a paternally-inherited gene. Further studies are needed to evaluate its role in human fertilization.

키워드

H19, imprinted; IGF2; IGF1R; Infertility, male; Oligozoospermia; Sperm count

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