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Comparison of Autophagy mRNA Expression between Chronic Otitis Media With and Without Cholesteatoma

Journal of Audiology & Otology 2020년 24권 4호 p.191 ~ 197
정준양, 정수영, 김명구, 김영일, 김상훈, 여승근,
소속 상세정보
정준양 ( Jung Jun-Yang ) - Kyung Hee University School of Medicine Department of Anatomy and Neurobiology
정수영 ( Jung Su-Young ) - Hanyang University College of Medicine Myongji Hospital Department of Otorhinolaryngology-Head and Neck Surgery
김명구 ( Kim Myung-Gu ) - Sungkyunkwan University School of Medicine Samsung Changwon Hospital Department of Otorhinolaryngology-Head and Neck Surgery
김영일 ( Kim Young-Il ) - Kyung Hee University Medical Center Medical Science Research Institute
김상훈 ( Kim Sang-Hoon ) - Kyung Hee University School of Medicine Department of Otorhinolaryngology
여승근 ( Yeo Seung-Geun ) - Kyung Hee University School of Medicine Department of Otorhinolaryngology

Abstract


Background and Objectives: Autophagy is known to be associated with pathogen infection. However, the expression of autophagy-related proteins has not been studied in chronic otitis media without cholesteatoma (COM) or with cholesteatoma (CholeOM). This study aimed to determine whether there is a difference between COM and CholeOM in autophagy-related gene mRNA expression.

Subjects and Methods: For 47 patients with chronic otitis media, the inflammatory tissues were classified into granulation tissue (COM) or cholesteatoma (CholeOM) according to biopsy results.

Results: PI3K mRNA expression (COM vs. CholeOM, mean±SD, 0.009±0.010 vs. 0.003±0.004; p=0.004) was lower, whereas Beclin-1 mRNA expression (0.089±0.107 vs. 0.176±0.163; p=0.034) was higher in the CholeOM group. Expression of PI3K mRNA in the CholeOM group was lower than that in the COM subgroups with presence of bacteria (0.022±0.019 vs. 0.001±0.001; p=0.001), otorrhea (0.049±0.068 vs. 0.003±0.004; p=0.004), and hearing loss over 40 dB (0.083±0.130 vs. 0.003±0.004; p=0.005).

Conclusions: The data suggested that different autophagy proteins play important roles in chronic otitis media according to the presence or absence of cholesteatoma.

키워드

Autophagy; Otitis media; Cholesteatoma

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