잠시만 기다려 주세요. 로딩중입니다.

False-Positive Mycobacterium tuberculosis Detection: Ways to Prevent Cross-Contamination

Tuberculosis and Respiratory Diseases 2020년 83권 3호 p.211 ~ 217
Asgharzadeh Mohammad, Ozma Mahdi Asghari, Rashedi Jalil, Poor Behroz Mahdavi, Agharzadeh Vahid, Vegari Ali, Shokouhi Behrooz, Ganbarov Khudaverdi, Ghalehlou Nima Najafi, Leylabadlo Hamed Ebrahmzadeh, Kafil Hossein Samadi,
소속 상세정보
 ( Asgharzadeh Mohammad ) - Tabriz University of Medical Sciences Faculty of Paramedicine Biotechnology Research Center
 ( Ozma Mahdi Asghari ) - Tabriz University of Medical Sciences Student Research Committee
 ( Rashedi Jalil ) - Tabriz University of Medical Sciences Faculty of Paramedicine Tuberculosis and Lung Diseases Research Center
 ( Poor Behroz Mahdavi ) - Tabriz University of Medical Sciences Faculty of Paramedicine Tuberculosis and Lung Diseases Research Center
 ( Agharzadeh Vahid ) - Tabriz University of Medical Sciences Hematology and Oncology Research Center
 ( Vegari Ali ) - Urmia University of Medical Sciences Faculty of Medicine Department of Medical Physics
 ( Shokouhi Behrooz ) - Tabriz University of Medical Sciences Connective Tissue Diseases Research Center
 ( Ganbarov Khudaverdi ) - Baku State University Department of Microbiology
 ( Ghalehlou Nima Najafi ) - Tabriz University of Medical Sciences Student Research Committee
 ( Leylabadlo Hamed Ebrahmzadeh ) - Tabriz University of Medical Sciences Student Research Committee
 ( Kafil Hossein Samadi ) - Tabriz University of Medical Sciences Drug Applied Research Center

Abstract


The gold standard method for diagnosis of tuberculosis is the isolation of Mycobacterium tuberculosis through culture, but there is a probability of cross-contamination in simultaneous cultures of samples causing false-positives. This can result in delayed treatment of the underlying disease and drug side effects. In this paper, we reviewed studies on false-positive cultures of M. tuberculosis. Rate of occurrence, effective factors, and extent of false-positives were analyzed. Ways to identify and reduce the false-positives and management of them are critical for all laboratories. In most cases, false-positive is occurring in cases with only one positive culture but negative direct smear. The three most crucial factors in this regard are inappropriate technician function, contamination of reagents, and aerosol production. Thus, to reduce false-positives, good laboratory practice, as well as use of whole-genome sequencing or genotyping of all positive culture samples with a robust, extra pure method and rapid response, are essential for minimizing the rate of false-positives. Indeed, molecular approaches and epidemiological surveillance can provide a valuable tool besides culture to identify possible false positives.

키워드

Mycobacterium tuberculosis; False-Positive Culture; Cross-Contamination; Genotyping

원문 및 링크아웃 정보

 

등재저널 정보