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Validation of Dual Energy X-Ray Absorptiometry and Nuclear Magnetic Resonance in the Analysis of Body Composition in Mice

Journal of Bone Metabolism 2020년 27권 4호 p.291 ~ 299
백경완, 김지석, 박진성, 김소정, 하용찬, 정옥이, 유준일,
소속 상세정보
백경완 ( Baek Kyung-Wan ) - Gyeongsang National University Department of Physical Education
김지석 ( Kim Ji-Seok ) - Gyeongsang National University Department of Physical Education
박진성 ( Park Jin-Sung ) - Gyeongsang National University Hospital Department of Orthopaedic Surgery
김소정 ( Kim So-Jeong ) - Gyeongsang National University Department of Convergence of Medical Sciences
하용찬 ( Ha Yong-Chan ) - Chung-Ang University College of Medicine Department of Orthopaedic Surgery
정옥이 ( Jeong Ok-Yi ) - Gyeongsang National University Department of Convergence of Medical Sciences
유준일 ( Yoo Jun-Il ) - Gyeongsang National University Hospital Department of Orthopaedic Surgery

Abstract


Background: As an instrument for measuring body composition in experimental animals, dual energy X-ray absorptiometry (DXA) is ideal for accuracy, cost, and measurement efficiency. However, there is too little insight into the effectiveness of the various aspects of applying DXA to experimental animals. We investigated whether to compare and verify the precision and accuracy of DXA and nuclear magnetic resonance (NMR) animal body composition analyzers.

Methods: We used 30 Institution of Cancer Research mice in the study. First, in order to evaluate the reproducibility of DXA and NMR, we did repeated measurements by repositioning each mouse in anesthesia and euthanasia states. Subsequently, the accuracy of each device was evaluated by comparing the weight measured before the experiment, the weight of the tissue extracted from the mice after the experiment, and the measured DXA and NMR. In addition, when measuring the body composition of animals, we compared the time and the measurable body composition parameters and summarized the advantages and disadvantages of the 2 devices.

Results: Compared to NMR, DXA had the advantage of a fast measurement of bone composition and rapid image analysis. In addition, DXA showed a higher correlation (>95%) with fat mass, lean mass baseline than did NMR (>85%).

Conclusions: In conclusion, DXA was confirmed to have higher precision and measurement accuracy than did NMR. Therefore, DXA is an effective method for evaluating the body composition of experimental animals.

키워드

Absorptiometry, photon; Body composition; Magnetic resonance spectroscopy; Mice

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