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Comparison of IRIS Iq200, UF-1000i, and Cobas u701 Module Automated Urine Sediment Analyzers

Laboratory Medicine Online 2020년 10권 4호 p.283 ~ 294
최현지, 양다해, 권선자, 강푸름, 박하성, 김태윤, 황현용,
소속 상세정보
최현지 ( Choi Hyun-Ji ) - Kosin University Gospel Hospital Department of Laboratory Medicine
양다해 ( Yang Da-Hae ) - Kosin University Gospel Hospital Department of Laboratory Medicine
권선자 ( Kwon Sun-Ja ) - Kosin University Gospel Hospital Department of Laboratory Medicine
강푸름 ( Kang Poo-Reum ) - Kosin University College of Medicine
박하성 ( Park Ha-Sung ) - Gangneung Asan Medical Center Department of Orthopedics
김태윤 ( Kim Tea-Yun ) - Kosin University Gospel Hospital Department of Internal Medicine
황현용 ( Hwang Hyun-Yong ) - Kosin University Gospel Hospital Department of Laboratory Medicine

Abstract


Background: We sought to compare the performance of three commercially available automated urine sediment analyzers that represent the current urine sediment analysis technology.

Methods: A total of 232 patient samples were analyzed using manual microscopy and three automated analyzers: IRIS Iq200 (Beckman Coulter, USA), UF-1000i (Sysmex, Japan), and Cobas u701 (Roche, Switzerland). We analyzed precision, linearity, carry-over, concordance rate, and agreement between the three analyzers and manual microscopy.

Results: The repeatability and within-laboratory precision showed results similar to those of previous studies. All analyzers showed excellent linearity. The carry-over rates were within 1%. The correlation coefficient (r) between the three analyzers and manual microscopy was good. Regarding red blood cell (RBC), the UF-1000i showed a better concordance rate (90.52%) with manual microscopy than the other two analyzers and the agreement was substantial for UF-1000i (κ=0.63) and IRIS Iq200 (κ=0.61). Regarding white blood cell (WBC), Cobas u701 showed the best concordance rate (96.55%) and the agreement was moderate for IRIS Iq200 (κ=0.57) and Cobas u701 (κ=0.56), and fair for UF-1000i (κ=0.47). Regarding epithelial cell (EPI), IRIS Iq200 showed the highest concordance rate (99.2%) and the agreement was moderate for IRIS Iq200 (κ=0.59) and Cobas u701 (κ=0.54), and fair for UF-1000i (κ=0.40).

Conclusions: IRIS Iq200 offered the best agreement with manual microscopy for WBC and EPI count, while UF-1000i showed a better agreement for RBC count. The agreement is insufficient for fully replacing the manual microscopy.

키워드

Automated urine sediment analyzers; IRIS Iq200; UF-1000i; Cobas u701

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