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Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

Applied Microscopy 2020년 50권 1호 p.26 ~ 26
강지훈, 강은혜, 윤영식, 지승묵, 윤인식, 여종석,
소속 상세정보
강지훈 ( Kang Ji-Hun ) - Yonsei University School of Integrated Technology
강은혜 ( Kang Eun-Hye ) - Yonsei University College of Medicine Department of Plastic and Reconstructive Surgery
윤영식 ( Yun Young-Shik ) - Yonsei University School of Integrated Technology
지승묵 ( Ji Seung-Muk ) - Yonsei University Yonsei Institute of Convergence Technology
윤인식 ( Yun In-Sik ) - Yonsei University College of Medicine Department of Plastic and Reconstructive Surgery
여종석 ( Yeo Jong-Souk ) - Yonsei University School of Integrated Technology

Abstract


The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000?nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

키워드

Cell behavior; Human adipose-derived stem cell; Scanning electron microscope; Polyurethane acrylate; Nanopillar

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