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Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of Streptomyces coelicolor A3(2)

Genomics & Informatics 2020년 18권 3호 p.28 ~ 28
Ferdous Nadim, Reza Mahjerin Nasrin, Emon Md. Tabassum Hossain, Islam Md. Shariful, Mohiuddin A. K. M., Hossain Mohammad Uzzal,
소속 상세정보
 ( Ferdous Nadim ) - Mawlana Bhashani Science and Technology University Faculty of Life Science Department of Biotechnology and Genetic Engineering
 ( Reza Mahjerin Nasrin ) - Mawlana Bhashani Science and Technology University Faculty of Life Science Department of Biotechnology and Genetic Engineering
 ( Emon Md. Tabassum Hossain ) - Mawlana Bhashani Science and Technology University Faculty of Life Science Department of Biotechnology and Genetic Engineering
 ( Islam Md. Shariful ) - Hokkaido University Laboratory of Reproductive and Developmental Biology
 ( Mohiuddin A. K. M. ) - Mawlana Bhashani Science and Technology University Faculty of Life Science Department of Biotechnology and Genetic Engineering
 ( Hossain Mohammad Uzzal ) - National Institute of Biotechnology Bioinformatics Division

Abstract


Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.

키워드

Streptomyces coelicolor; genome; hypothetical protein; modeling; hydrolases

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