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Antioxidant Activity and Anti-Adipogenic Effects of Acai Berry (Euterpe oleracea Mart.) Juice and Extracts
±¸À²¸®, È«ÁÖÇö,
¼Ò¼Ó »ó¼¼Á¤º¸
±¸À²¸® ( Gu Yul-Ri ) - Daegu Catholic University Department of Food Science and Technology
È«ÁÖÇö ( Hong Joo-Heon ) - Daegu Catholic University Department of Food Science and Technology
Abstract
º» ¿¬±¸´Â ¾Æ»çÀ̺£¸® ÂøÁó¾×°ú ÃßÃâ¹°ÀÇ Ç×»êÈ È°¼º ¹× Áö¹æ¼¼Æ÷ºÐÈ ¾ïÁ¦ È¿°ú¸¦ È®ÀÎÇϱâ À§ÇØ ÀÌÈÇÐÀû ǰÁú Ư¼º, Ç×»êÈ È°¼º, L-132 ¹× 3T3-L1 ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ »ý¸®È°¼ºÀ» Á¶»çÇÏ¿´´Ù. ¾Æ»çÀ̺£¸® ÂøÁó¾×°ú ÃßÃâ¹°ÀÇ ÃÑ Æú¸®Æä³î, ÃÑ Çö󺸳ëÀ̵å, ÃÑ ´ç ¹× ÃÑ ¾ÈÅä½Ã¾Æ´Ñ ÇÔ·®Àº °¢°¢ 9.31~35.04 g/100 g, 9.30~26.13 g/100 g, 12.58~36.82 g/100 g ¹× 26.54~141.73 mg/L·Î ³ªÅ¸³µÀ¸¸ç, ÃÑ Æú¸®Æä³î, ÃÑ Çö󺸳ëÀÌµå ¹× ÃÑ ´ç ÇÔ·®Àº ¾Æ»çÀ̺£¸® ¿¼ö ÃßÃâ¹°¿¡¼ °¡Àå ³ôÀº ÇÔ·®À» ³ªÅ¸³Â°í ÃÑ ¾ÈÅä½Ã¾Æ´Ñ ÇÔ·®Àº ¾Æ»çÀ̺£¸® ¿¡Åº¿Ã ÃßÃâ¹°¿¡¼ °¡Àå ³ôÀº ÇÔ·®À» ³ªÅ¸³»¾ú´Ù. Ç×»êÈ È°¼º ¹× È¿¼Ò ÀúÇØ Ȱ¼ºÀº ¸ðµÎ 1,000 ¥ìg/mL ³óµµ¿¡¼ ¾Æ»çÀ̺£¸® ¿¼ö ÃßÃâ¹°ÀÌ °¡Àå ¿ì¼öÇÏ°Ô ³ªÅ¸³µÀ¸¸ç, L-132 ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ »êÈÀû ¼Õ»ó¿¡ ´ëÇÑ ¼¼Æ÷Àç»ý È¿°ú´Â ¾Æ»çÀ̺£¸® ¿¼ö ÃßÃâ¹°(1,000 ¥ìg/mL)¿¡¼ 93.93 ¡¾0.74%·Î °¡Àå ¿ì¼öÇÑ ¼¼Æ÷Àç»ý È¿°ú¸¦ È®ÀÎÇÏ¿´´Ù. 3T3- L1 ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ Áß¼ºÁö¹æ ÇÔ·® ¹× free glycerol ÇÔ·® ¶ÇÇÑ ¾Æ»çÀ̺£¸® ¿¼ö ÃßÃâ¹°(1,000 ¥ìg/mL)¿¡¼ °¢°¢ 58.08 ¡¾0.81%, 34.86¡¾0.02%·Î °¡Àå ³·Àº Áö¹æ±¸ ¿°»ö ¹× free glycerol ÇÔ·®À» È®ÀÎÇÏ¿´´Ù. 3T3-L1 ¼¼Æ÷ ³» Æ÷µµ´ç Èí¼ö´ÉÀº Àν¶¸° ¹«Ã³¸®±ºº¸´Ù Àν¶¸° 󸮱º¿¡¼ ´õ ³ôÀº Æ÷µµ´ç Èí¼ö´ÉÀ» ³ªÅ¸³Â°í, Àν¶¸° 󸮱º Áß ¾Æ»çÀ̺£¸® ¿¼ö ÃßÃâ¹°(1,000 ¥ìg/mL)¿¡¼ 85.86¡¾0.58%·Î °¡Àå ³ôÀº Æ÷µµ´ç Èí¼ö´ÉÀ» ³ªÅ¸³Â´Ù. µû¶ó¼ ¾Æ»çÀ̺£¸® ÂøÁó¾× ¹× ÃßÃâ¹°ÀÇ ´Ù¾çÇÑ ¼¼Æ÷ ³» ±âÀüÀ» ÅëÇØ Ç×»êÈ È°¼º ¹× Áö¹æ¼¼Æ÷ºÐÈ ¾ïÁ¦ È¿°ú°¡ ÀÖÀ½À» È®ÀÎÇÏ¿´À¸¸ç ¾Æ»çÀ̺£¸® ÂøÁó¾× ¿ª½Ã ÇâÈÄ Ç×»êÈ ¹× Ç׺ñ¸¸ ±â´É¼º ½Äǰ ¼ÒÀç·Î Ȱ¿ëµÉ ¼ö ÀÖÀ» °ÍÀ¸·Î »ç·áµÈ´Ù.
This study evaluated the antioxidant and anti-obesity activities of acai berry juice and extracts. The total polyphenol, total flavonoid, and total sugar contents of AHE (hot-water extract of acai berry) were 35.04 g/100 g, 26.13 g/100 g, and 36.82 g/100 g, respectively. The total anthocyanin content of AEE (70% ethanol extract of acai berry) was 141.73 mg/L. The DPPH, ABTS radical scavenging activity, and FRAP activity of AHE at 1,000 ¥ìg/mL were 59.40%, 83.10%, and 2.03 mM, respectively. The ¥á-amylase, ¥á-glucosidase, and lipase inhibitory activity of AHE at 1,000 ¥ìg/mL were 84.03%, 92.10%, and 31.85%, respectively. AHE had better regeneration effects against oxidative stress in L-132 cells than the other extracts. Furthermore, AHE suppressed adipocyte differentiation, lipid accumulation, and the free glycerol content, and triggered lipolysis on MDI treated 3T3-L1 preadipocytes. Thus, in addition to AHE and AEE, AJ also has antioxidant activity and inhibits adipocyte in 3T3-L1 cells, resulting in reduced lipid accumulation, reduced free glycerol release, and increased glucose uptake.
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acai berry; antioxidant; anti-adipogenic; juice; extracts
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