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한국의 한 대학병원에서 수집된 Acinetobacter baumannii 임상분리주의 meropenem 내성에 대한 AdeABC 유출펌프의 역할

The role of AdeABC efflux pump to meropenem resistance in Acinetobacter baumannii clinical isolates collected from a university hospital in Korea

The Medical Journal of Chosun University 2020년 45권 2호 p.75 ~ 79
심재광, 최지애, 김춘미, 고영진, 강성호, 박건, 장숙진,
소속 상세정보
심재광 ( Shim Jae-Kwang ) - Chosun University Hospital Department of Laboratory Medicine
최지애 ( Choi Ji-Ae ) - Centers for Disease Control and Prevention National Institute of Health Division of Antimicrobial Resistance
김춘미 ( Kim Choon-Mee ) - Chosun University College of Medicine Premedical Science
고영진 ( Ko Young-Jin ) - Chosun University College of Medicine Department of Laboratory Medicine
강성호 ( Kang Seong-Ho ) - Chosun University College of Medicine Department of Laboratory Medicine
박건 ( Park Geon ) - Chosun University College of Medicine Department of Laboratory Medicine
장숙진 ( Jang Sook-Jin ) - Chosun University College of Medicine Department of Laboratory Medicine

Abstract


The aim of this study is to investigate the role of the AdeABC efflux pump to meropenem resistance in 116 clinical isolates of Acinetobacter baumannii collected from a university hospital in Korea. We performed real-time RT-PCR to test the relative expression of adeB efflux pumps gene in them. The ratio of meropenem MIC change due to carbonyl cyanide m-chlorophenylhydrazone (CCCP), efflux pump inhibitor, was calculated. It was judged that the efflux pump contributed to meropenem resistance when the changed ratio of meropenem MIC by CCCP was 4 or more. The mean and standard error of the adeB relative expression level in the susceptible and resistant groups to meropenem were 3.2±0.8 and 11.9±2.9, respectively and there was a significant difference between the two groups (p<0.0001). The ratio of meropenem MIC changes due to CCCP to 4 or higher in the susceptible and resistant groups to meropenem were 17.4% and 20.4%, respectively, and there was no significant difference between them. No correlation was observed between the adeB expression and the ratio of meropenem MIC change due to CCCP. It is considered that the adeB efflux pump did not significantly affect meropenem resistance in most strains of A. baumannii tested in this study.

키워드

Acinetobacter baumannii; adeB protein; Bacterial; Drug resistance; Meropenem

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