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위령탕 혼합단미연조엑스의 항염증 효과

Anti-inflammatory Effects of Wiryeong-tang Mix Soft Extract

대한한의학방제학회지 2020년 28권 4호 p.339 ~ 349
김명진, 김세진, 남원희, 임현희, 전영희, 손수미, 김정옥,
소속 상세정보
김명진 ( Kim Myoung-Jin ) - National Institute for Korean Medicine Development
김세진 ( Kim Se-Jin ) - National Institute for Korean Medicine Development
남원희 ( Nam Won-Hee ) - National Institute for Korean Medicine Development
임현희 ( Leem Hyun-Hee ) - National Institute for Korean Medicine Development
전영희 ( Jeon Young-Hee ) - National Institute for Korean Medicine Development
손수미 ( Son Su-Mi ) - National Institute for Korean Medicine Development
김정옥 ( Kim Jung-Ok ) - National Institute for Korean Medicine Development

Abstract


Objectives : Wiryeong-tang (WRT) is a traditional herbal medicine used to treat kidney-related diseases. However, the anti-inflammatory and anti-gastritis effect of Wiryeong-tang was not well known. Therefore, we experimented to confirmed the anti-inflammatory and anti-gastritis effects of Wiryeong-tang.

Methods : The RAW 264.7 cells were pre treated with Wiryeong-tang mix soft extract (WRT-mse; 50, 100 and 200 ㎍/mL) for 1 hrs, and then incubated with lipopolysaccharide (LPS; 500 ng/mL). Cell viability was measured by the MTT method, and nitric oxide (NO) was measured with griess reagent. In addition, pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). For anti-gastritis effect in vivo, acute gastritis was induced using 150 mM HCl/60% ethanol used ICR mice. WRT-mse (133 mg/kg) was pre treated for 3 days and then treated with 150 mM HCl/60% ethanol 1 hrs later. Then gastritis was observed and inflammatory cytokines in the gastric tissue was measured.

Results : The 8 marker components of the WRT-mse were determined by simultaneous analysis using HPLC. WRT-mse was not toxic and inhibited pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α at NO production, protein and mRNA levels. Also, it was confirmed that WRT-mse improved bleeding and edema in gastritis, and suppresses inflammatory cytokines.

Conclusion : In summary, our results suggest that the treatment of the WRT-mse reduced and improved the 150 mM HCl/60% ethanol induced acute gastritis and the inflammation caused by LPS stimulation in RAW 264.7 cells. Therefore, this study may provide useful drug or clinical evidence for WRT-mse to prevent inflammation.

키워드

Wiryeong-tang mix soft extract (WRT-mse); lipopolysaccharide (LPS); acute gastritis; pro-inflammatory cytokines; anti-inflammatory; anti-gastritis

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