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Alterations in Growth and Morphology of Ganoderma lucidum and Volvariella volvaceae in Response to Nanoparticle Supplementation

Mycobiology 2020년 48권 5호 p.383 ~ 391
Singh Swarnjeet, Kuca Kamil, Kalia Anu,
소속 상세정보
 ( Singh Swarnjeet ) - Punjab Agricultural University College of Basic Sciences and Humanities
 ( Kuca Kamil ) - University of Hradec Kralove Faculty of Science Department of Chemistry
 ( Kalia Anu ) - Punjab Agricultural University College of Agriculture Department of Soil Science

Abstract


Use of nanoparticles (NPs) in several commercial products has led to emergence of novel contaminants of air, soil and water bodies. The NPs may exhibit greater ecotoxicity due to nano-scale dependent properties over their bulk counterparts. The present investigation explores the effect of in vitro supplementation of TiO2, silica and silver NPs on radial growth and ultrastructural changes in the hyphae and spores of two mushroom genera, Ganoderma lucidum and Volvariella volvaceae. A concentration dependent decrease in radial growth on NP amended potato dextrose agar medium was recorded. However, in comparison to control, there was decrease in radial diameter on supplementation with TiO2 NPs while an increase was recorded for silica and silver NPs amendments as compared to their bulk salts at same concentrations after 48?h of incubation. Optical microscopy studies showed decrease in the number of spores while increase in spore diameter and thinning of hyphal diameter on NPs supplementation. Scanning electron microscopy analysis of fungal growth showed presence of deflated and oblong spores in two fruiting strains of Ganoderma while Volvariella exhibited decreased sporulation. Further, hyphal thinning and branching was recorded in response to NP amendments in both the test mushrooms. Enhancement of protein content was observed on NP compared to bulk supplementation for all cultures, concentrations and hours of incubation except for TiO2 NPs. Likewise, bulk and NP supplementations (at 100?mg L ?1) resulted in enhanced laccase activity with occurrence of laccase specific protein bands on SDS-PAGE analysis.

키워드

Laccase enzyme; nanoparticles; protein content; radial diameter

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