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Fermented Non-Digestible Fraction of Andean Berry (Vaccinium meridionale Swartz) Juice Induces Apoptosis in Colon Adenocarcinoma Cells

Preventive Nutrition and Food Science 2020년 25권 3호 p.272 ~ 279
Agudelo Carlos Daniel, Luzardo-Ocampo Ivan, Hernandez-Arriaga Angelica Maria, Rendon Julio Cesar, Campos-Vega Rocio, Maldonado-Celis Maria Elena,
소속 상세정보
 ( Agudelo Carlos Daniel ) - University of Antioquia School of Nutrition and Dietetics
 ( Luzardo-Ocampo Ivan ) - Autonomous University of Queretaro School of Chemistry Research and Graduate Studies in Food Science
 ( Hernandez-Arriaga Angelica Maria ) - Autonomous University of Queretaro School of Chemistry Research and Graduate Studies in Food Science
 ( Rendon Julio Cesar ) - University of Antioquia School of Nutrition and Dietetics
 ( Campos-Vega Rocio ) - Autonomous University of Queretaro School of Chemistry Research and Graduate Studies in Food Science
 ( Maldonado-Celis Maria Elena ) - University of Antioquia School of Nutrition and Dietetics

Abstract


Vaccinium meridionale Swartz, known as Andean berry, has a high content of anthocyanins, phenolic acids, and other flavonoids due to their putative anticancer activity. However, after consumption, the structures and function of these molecules may be altered. The purpose of this study was to evaluate the pro-apoptotic effect of fermented non-digestible fraction (FNDF) of Andean berry juice (ABJ) on colon adenocarcinoma HT29 cells. HT29 cells were treated by FNDF-ABJ obtained by in vitro gastrointestinal fermentation. We determined the proapoptotic capacity by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, oxidative stress by analyzing superoxide dismutase and catalase activity, lipid peroxidation by measuring 8-iso-prostaglandin F2α, and measured lactate dehydrogenase. Our results show that FNDF-ABJ inhibited cell growth [lethal dose 50(%)=26% v/v]. In addition, FNDF-ABJ increased the number of TUNEL positive cells 2-fold compared with untreated cells without altering the release of lactate dehydrogenase. However, superoxide dismutase activity was reduced in HT29 cells treated with FNDF-ABJ, catalase activity was not affected and 8-iso-prostaglandin F2α levels were increased. These results support that the anti-proliferative effects of FNDF-ABJ on HT29 cells can be explained by apoptotic mechanisms.

키워드

apoptosis; colon cancer; oxidative stress; Vaccinium

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