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Evaluation of the Phenolic Content, Antioxidant and Antimicrobial Activities of Oil and Non-Oil Extracts of Citrus sinensis (L.) Osbeck Seeds

Preventive Nutrition and Food Science 2020년 25권 3호 p.280 ~ 285
Oikeh Ehigbai I., Ayevbuomwan Merit, Irabor Francis, Oikeh Aiemere O., Oviasogie Faith E., Omoregie Ehimwenma S.,
소속 상세정보
 ( Oikeh Ehigbai I. ) - University of Benin Faculty of Life Sciences Department of Biochemistry
 ( Ayevbuomwan Merit ) - University of Benin College of Basic Medical Sciences Department of Medical Biochemistry
 ( Irabor Francis ) - Michael and Cecilia Ibru University Faculty of Natural and Applied Sciences Department of Biological Sciences
 ( Oikeh Aiemere O. ) - University of Benin Faculty of Life Sciences Department of Biochemistry
 ( Oviasogie Faith E. ) - University of Benin Faculty of Life Sciences Department of Microbiology
 ( Omoregie Ehimwenma S. ) - University of Benin Faculty of Life Sciences Department of Biochemistry

Abstract


The seeds of Citrus sinensis (L.) Osbeck (sweet orange) are waste products usually discarded. They may however contain phytochemicals that have potent bioactivities. In this study, the phenolic content, and antioxidant and antimicrobial activities of oil and non-oil (solid) extracts of C. sinensis seeds were evaluated using standard protocols. The seed oil contained significantly (P>0.05) higher contents of total phenol and total flavonoid when compared to the solid extract. However, the non-oil extract contained significantly (P<0.05) higher tannin contents than the seed oil. Ferric reducing antioxidant potential was not significantly different between both extracts. The antimicrobial activities of both extracts revealed that the seed oil possesses better antibacterial activities compared to the non-oil extract. The antifungal test revealed that the seed oil significantly inhibited the growth of Candida albicans (20 mm zone of inhibition at a concentration of 200 μg/mL), however, it did not inhibit the growth of Aspergillus niger and Penicillum sp. The minimum inhibitory concentration values against the bacterial and fungal strains were similar for both extracts in the range of 50∼100 μg/mL. Minimum bactericidal concentration and minimum fungicidal concentration values ranged from 100∼200 μg/mL for both extracts. The results in this study indicate that C. sinensis seed oil and non-oil extracts possess antioxidant, and antibacterial and antifungal properties that may be differentially exploited in the development of antimicrobial agents.

키워드

antimicrobial; Citrus sinensis; minimum bactericidal concentration; minimum fungicidal concentration; minimum inhibitory concentration

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