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Canine amniotic membrane derived mesenchymal stem cells exosomes addition in canine sperm freezing medium

한국동물생명공학회지 2020년 35권 3호 p.268 ~ 272
Mahiddine Feriel Yasmine, Qamar Ahmad Yar, 김민정,
소속 상세정보
 ( Mahiddine Feriel Yasmine ) - Seoul National University College of Veterinary Medicine Department of Theriogenology and Biotechnologies
 ( Qamar Ahmad Yar ) - Chungnam National University College of Veterinary Medicine
김민정 ( Kim Min-Jung ) - Seoul National University College of Veterinary Medicine Department of Theriogenology and Biotechnologies

Abstract


Amniotic membrane stem cells are considered as a good alternative to embryonic stem cells, but their use in clinical studies is still not common. Here, exosomes from canine amniotic membrane mesenchymal stem cells (cAmMSCexo) were used for dog sperm cryopreservation. Upon cryopreserved straws using cryoprotectant containing 0, 0.5, 1, or 2 μg/mL of cAmMSC-exo were thawed, motility and membrane integrity were analyzed. However, results showed no significant differences between the groups. We concluded that cAmMSC-exo with lower than 2 μg/mL have no effects on sperm cryopreservation, and further studies to get higher concentrations of cAmMSC-exo should be conducted for clinical application.

키워드

amniotic membrane; dog sperm; exosomes; mesenchymal stem cells; sperm cryopreservation

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