Roles of transcription factor binding sites in the D-raf promoter region
권은정, 김형인, 김인주, 유미애,
소속 상세정보
권은정 ( Kwon Eun-Jeong ) - Pusan National University Department of Molecular Biology
김형인 ( Kim Hyeong-In ) - Pusan National University Department of Molecular Biology
김인주 ( Kim In-Ju ) - Pusan National University Department of Molecular Biology
유미애 ( Yoo Mi-Ae ) - Pusan National University Department of Molecular Biology
Abstract
D?raf, a Drosophila homolog of the human c?raf?1, is known as a signal transducer in cell proliferation and differentiation. A previous study found that the D?raf gene expression is regulated by the DNA replication?related element (DRE)/DRE?binding factor (DREF) system. In this study, we found the sequences homologous to transcription factor C/EBP, MyoD, STAT and Myc recognition sites in the D?raf promoter. We have generated various base substitutional mutations in these recognition sites and subsequently examined their effects on D?raf promoter activity through transient CAT assays in Kc cells with reporter plasmids p5'?878DrafCAT carrying the mutations in these binding sites. Through gel mobility shift assay using nuclear extracts of Kc cells, we detected factors binding to these recognition sites. Our results show that transcription factor C/EBP, STAT and Myc binding sites in D?raf promoter region play a positive role in transcriptional regulation of the D?raf gene and the Myo D binding site plays a negative role
키워드
Drosophila; Raf; Promoter; Transcription factor binding site
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