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Characteristics of (3H)-choline uptake into synaptosomes from rat hippocampus

Animal Cells and Systems 1998년 2권 2호 p.233 ~ 238
이서은, 홍승길, 최임순,
소속 상세정보
이서은 ( Lee Seo-Eun ) - Korea University College of Medicine Department of Physiology
홍승길 ( Hong Seung-Kil ) - Korea University College of Medicine Department of Physiology
최임순 ( Choe Rim-Soon ) - Yonsei University College of Natural Science Department of Biology

Abstract


Certain basic characteristics of choline uptake in nerve terminals were studied with synaptosomes from rat hippocampus. Synaptosomal [3H]?choline uptake was clarified as specific and high affinity by low Km value (2.2 μM), Na+?dependency and high sensitivity to hemicholinium?3, a competitive inhibitor of choline uptake. Choline uptake into synaptosomes was linearly related to Na+ concentration and membrane potential. Extracellular Ca2+ modulated the choline uptake, but probably not through increase of intracellular Ca2+ because this modulation was not affected the by high K+?depolarization. EGTA (2 mM) added for Ca2+ ?free condition had a peculiar effect of decreasing choline uptake. These results suggest that Ca2+ may play an important role in regulating the metabolism of acetylcholine in the nerve terminals directly through the increase of acetylcholine release

키워드

Calcium; Choline uptake; High affinity; Na+-dependent; Synaptosomes

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