Identification of three types of voltage dependent Ca2+-channels in mouse follicular oocytes
배인하, 윤숙영, 윤용달, 김문규, 김해권,
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배인하 ( Bae In-Ha ) - Sungshin Women’s University College of Natural Sciences Department of Biology
윤숙영 ( Yoon Sook-Young ) - Sungshin Women’s University College of Natural Sciences Department of Biology
윤용달 ( Yoon Yong-Dal ) - Hanyang University College of Natural Sciences Department of Biology
김문규 ( Kim Moon-Kyoo ) - Hanyang University College of Natural Sciences Department of Biology
김해권 ( Kim Hae-kwon ) - Seoul Women’s University College of Natural Sciences Department of Biology
Abstract
The immunocytochemical method was used to identify the existence of voltage?dependent Ca2+?channels in mouse follicular oocytes. Three types of voltage?dependent Ca2+?channels were shown to exist in the follicular oocytes for the first time, the P/Q?type Ca2+?channel, the N?type Ca2+?channel, and the L?type Ca2+?channel. Among proven Ca2+ ?channels, distributions of the P/Q?type Ca2+?channel and L?type Ca2+ ?channel showed localized staining (clustered pattern) on the oolemma. The distribution of the P/Q?type Ca2+ ?channel showed all localized staining, and the range of localized staining was from 1 to 8 in staining intensity. As the staining intensity increased from 1 to 8, the number of localized staining decreased. The L?type Ca2+?channel are homogeneously stained (29.4%?54.2%), while some of them (around 28.7%?44.1%) showed localized staining on the oolemma. However, the rest of them showed no staining at all (17.1%? 26.5%). On the contrary, the N?type Ca2+?channel showed mostly homogeneous staining, while non?staining oocytes were around 33.8%. The rest showed localized staining (10%). However, staining intensity was much weaker than those of the P/Q?type and L?type Ca2+ ?channel. In fact, the N?type Ca ?channel has been known to exist only in neurons (from ectoderm origin), but it is unknown how the N?type Ca2+ ?channel exists in the follicular oocytes (from mesoderm origin). Further studies are needed to examine the expression of Ca2+?channels during the developmental stages of the oocytes
키워드
Mouse follicular oocyte; Ca2+-channel; P/Q-type Ca2+-channel; N-type Ca2+-channel; L-type Ca2+-channel
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