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Identification of a tandemly repeated DNA sequence using combined RAPD and FISH in welsh onion (allium Fistulosum)

Animal Cells and Systems 1999년 3권 1호 p.69 ~ 72
서봉보, 도금숙, 이선희,
소속 상세정보
서봉보 ( Seo Bong-Bo ) - Kyungpook National University College of National Sciences Department of Biology
도금숙 ( Do Geum-Sook ) - Kyungpook National University College of National Sciences Department of Biology
이선희 ( Lee Seon-Hee ) - Kyungpook National University College of National Sciences Department of Biology

Abstract


A tandemly repeated DNA sequence was identified and characterized by the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5?GAAACGGGTG?3’, in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C?banded constitutive heterochromatin in the terminal regions of both arms of all 16 chromosomes

키워드

Tandemly repeated DNA sequence; RAPD; FISH; Nucleotide sequence

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