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Activation of PKC-βII is required for vitamin e-succinate-induced apoptosis of U937 cells

Animal Cells and Systems 2000년 4권 3호 p.279 ~ 285
김송자, 박재한, 이선령, 방옥선, 강신성,
소속 상세정보
김송자 ( Kim Song-Ja ) - Kyungpook National University College of Natural Sciences Department of Biology
박재한 ( Park Jae-Han ) - Kyungpook National University College of Natural Sciences Department of Biology
이선령 ( Lee Sun-Ryung ) - Kyungpook National University College of Natural Sciences Department of Biology
방옥선 ( Bang Ok-Sun ) - Kyungpook National University College of Natural Sciences Department of Biology
강신성 ( Kang Shin-Sung ) - Kyungpook National University College of Natural Sciences Department of Biology

Abstract


Vitamin E?succinate (VES) treatment of U937 human monoblasts induced cells to undergo apoptosis. After 96 h of VES treatment at 10μg/ml, more than 80% of cells appeared apoptotic. Evidence for apoptosis by VES was based on propidium iodide staining for detection of chromatin condensational fragmentation and electrophoretic DNA ladder formation. Western blot analyses showed a transient increase in Fas and p21 protein levels up to 48 h after the VES treatment. Protein expression and activity of CDK1 and lamin B degradation were remarkably induced by VES, following the cleavage of caspase?3 after 48 h. The VES?induced apoptosis was found to involve activation of PKC as shown by increases in membrane translocation of PKCPII and PKC activity. Pretreatment of GF109203X (PKC inhibitor) prior to VES treatment almost completely inhibited the induction of apoptosis as assessed by blockage of VES?induced caspase?3 activity and DNA fragmentation. However, GF109203X had no effect on the VES?induced nitric oxide synthesis, which was required for monocytic differentiation in our previous report (J Cell Sci 111, 435, 1998). Taken together, our data suggest that induction of apoptosis by VES in U937 cells occurs through activation of PKC?βII resulting in the activation of caspase?3 cascade and is independent of nitric oxide.

키워드

Apoptosis; Caspase; Fas; PKC; Vitamin E-succinate

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