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Effect of low dose mutagens on adaptive response and plasma membrane glycoconjugates in sarcoma 180 cells

Animal Cells and Systems 2000년 4권 3호 p.293 ~ 297
이종화, 최은실, 오규선, 이동욱, 장정현, 엄경일,
소속 상세정보
이종화 ( Lee Jong-Hwa ) - Dong-A University Division of Natural Sciences
최은실 ( Choi Eun-Sil ) - Dong-A University Division of Natural Sciences
오규선 ( Oh Kyu-Seon ) - Dong-A University Division of Natural Sciences
이동욱 ( Lee Dong-Wook ) - Dong-A University Division of Natural Sciences
장정현 ( Chang Jeong-Hyun ) - Dong-A University Division of Natural Sciences
엄경일 ( Um Kyung-II ) - Dong-A University Division of Natural Sciences

Abstract


The present investigation was performed to elucidate the effect of pretreat?ment with low dose ultraviolet radiation (UV) and ethyl methanesulfonate (EMS) on cell survival by trypan blue dye exclusion method and plasma membrane glycoconjugates by lectin?cytochemistry in sarcoma 180 (S180) cells. Pretreatment with 2 J/m2 UV or 2 mM EMS increased the percentage of survival of cells subsequently treated with high dose UV (10 or 20 J/m2) or EMS (10 or 20 mM), respectively. Staining intensity of concanavalin A (Con A) of the cells pretreated with 2 J/m UV or 2 mM EMS and subsequently treated with 10 or 20 mM EMS was stronger than that of the cells treated with 10 or 20 mM EMS. These results suggest that there is an adaptive response on cell survival to EMS or UV in S180 cells. And the results show a change in mannose?containing glycoconjugates of plasma membrane in S180 cells pretreated with EMS or UV and subsequently treated with EMS.

키워드

Adaptive response; Concanavalin A; Glycoconjugates; Mannose; S180 cells

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