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Ultrastructure of the follicular oocyte surface in Rana dybowskii

Animal Cells and Systems 2001년 5권 1호 p.45 ~ 50
주정원, 임욱빈, 권혁방, 최형식,
소속 상세정보
주정원 ( Ju Jung-Won ) - Chonnam National University Hormone Research Center
임욱빈 ( Im Wook-Bin ) - Chonnam National University Hormone Research Center
권혁방 ( Kwon Hyuk-Bang ) - Chonnam National University Hormone Research Center
최형식 ( Choi Hueng-Sik ) - Chonnam National University Hormone Research Center

Abstract


Rana ovarian follicles consist of oocyte, vitelline envelope, granulosa cells, and theca/epithelial layer. Using scanning electron microscopy, the surface structure of each follicular component was investigated. Changes in oocyte surface during oocyte maturation were also examined. Theca/epithelial layer was almost transparent and some blood vessels and granulosa cells were observed underneath in intact follicle. The number of granulosa cells was estimated to be 6700?7200 per oocyte. The granulosa cells partially overlapped each other and their microvilli penetrated the vitelline membrane via holes present in the vitelline envelope and seemed to be linked to oocyte microvilli. After removal of the vitelline envelope by microforcep, oocyte microvilli were observed on the surface of the devitellined oocyte. The oocyte microvilli formed partial clusters on the surface of white spot area which appears just before germinal vesicle breakdown (GVBD), whereas they were evenly distributed in other areas. The microvilli became shorter and less dense with oocyte maturation. The lengths of oocyte microvilli in the immature and mature oocyte were 1.5 μm and 0.6 μm, respectively. The present study suggests a fundamental structural change occurring on the oocyte surface during maturation.

키워드

Meiotic maturation; Oocyte; Oocyte surface; Amphibian

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