Identification and phytogeny of the human endogenous retrovirus HERV-W LTR Family in Cancer Cells
이주미, 김환묵, 김희수,
소속 상세정보
이주미 ( Yi Joo-Mi ) - Pusan National University College of Natural Sciences Division of Biological Sciences
김환묵 ( Kim Hwan-Mook ) - Korea Research Institute of Bioscience and Biotechnology
김희수 ( KIM HEUI-SOO ) - Pusan National University College of Natural Sciences Division of Biological Sciences
Abstract
The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV?W LTR elements in various cancer cells (2F7, A431, A549, HepG2, MIA?PaCa?2, PC?3, RT4, SiHa, U?937, and UO?31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV?W LTR elements. Those LTR elements showed a high degree of sequence similarity (88?99%) with HERV?W LTR (A F072500). A phylogenetic tree obtained by the neighbor?joining method revealed that HERV?W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV?W LTR elements (RT4?2, A431?1, and UO31?2) belonged to Group I, whereas nine LTR elements (2F7?2, A549?1, A549?3, HepG2?3, MP2?2, PC3?1, SiHa?8, SiHa?10, and U937?1) belonged to Group D. Taken together, our new sequence data of the HERV?W LTR elements may contribute to an understanding of tissue?specific cancer by genomic instability of LTR integration.
키워드
Cancer cells; HERV?W; LTR elements; Phylogeny
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