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Trigger factor interacts with DnaA protein to stimulate its interaction with DnaA box

Animal Cells and Systems 2003년 7권 1호 p.81 ~ 87
이용선, 이준, 김학균, 강석현, 한주석, 김재범, 황덕수,
소속 상세정보
이용선 ( Lee Yong-Sun ) - Seoul National University School of Biological Sciences
이준 ( Lee June ) - Seoul National University School of Biological Sciences
김학균 ( Kim Hak-Kyun ) - Seoul National University School of Biological Sciences
강석현 ( Kang Suk-Hyun ) - Seoul National University School of Biological Sciences
한주석 ( Han Joo-Seok ) - Seoul National University School of Biological Sciences
김재범 ( Kim Jae-Bum ) - Seoul National University School of Biological Sciences
황덕수 ( Hwang Deog-Su ) - Seoul National University School of Biological Sciences

Abstract


While screening proteins that interact with DnaA protein, the initiator protein for Escherichia coli chromosomal DNA replication, we found a 52?kD sized protein which bound to DnaA protein in a salt?dependent manner. This protein was identified as trigger factor, a ribosome?associated peptidyl?prolyl?cis/trans isomerase with chaperone activity. Trigger factor was overproduced and purified to near homogeneity, and its effect on the function of DnaA protein was examined. Enhanced binding of DnaA protein to DnaA box with no apparent super shift in the gel?shift experiments suggested that trigger factor, by virtue of its chaperone activity, exerts a change on DnaA protein thus increasing its binding affinity for DnaA box.

키워드

DnaA protein; Trigger factor; DnaA box; Chaperone

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