Caspase-11 promoter-GFP construct as a dual reporter of cytotoxicity and inflammation
신기순, 강신정,
소속 상세정보
신기순 ( Shin Ki-Soon ) - Kyung Hee University Department of Biology
강신정 ( Kang Shin-Jung ) - Sejong University Department of Molecular Biology
Abstract
Caspase?11 has been known as a dual regulator of apoptosis and inflammatory response. An unusual feature of caspase?11 is that its expression is induced by apoptotic or proinflammatory stimuli. Utilizing these unusual features of caspase?11, we have developed a simple and sensitive assay method to screen pro? or anti?apoptotic/inflammatory molecules. To develop this assay method, we generated a reporter construct where GFP expression is regulated by caspase?11 promoter. When several types of cultured cells were transfected with this reporter construct and subsequently treated with various apoptotic or proinflammatory molecules, expression of GFP by the activation of caspase?11 promoter was easily detected by fluorescence microscopy or spectro?fluorometry. In addition, a reduction of the GFP fluorescence was detected when an agent reported to suppress caspase?11 induction was applied. These results suggest that our reporter system can be used to screen pro? or anti?apoptotic/ inflammatory molecules.
키워드
Caspase-11 promoter-GFP; cytotoxicity; inflammation
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