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Genetic screening of the Dazl?interacting protein genes

Animal Cells and Systems 2006년 10권 4호 p.227 ~ 231
이경호, 이성주, 이건수,
소속 상세정보
이경호 ( Lee Kyung-Ho ) - Seoul National University College of Natural Sciences Department of Biological Sciences
이성주 ( Lee Seong-Ju ) - Seoul National University College of Natural Sciences Department of Biological Sciences
이건수 ( Rhee Kun-Soo ) - Seoul National University College of Natural Sciences Department of Biological Sciences

Abstract


Micro?deletions at specific loci of the Y chromosome have been observed frequently in male infertility patients, suggesting that genes in these regions are involved in male germ cell development. DAZ is a representative male infertility gene at the AZFc locus of the Y chromosome. Since DAZ contains an RNA binding motif along with so?called a DAZ domain, it was proposed to participate in RNA metabolism during spermatogenesis. A mouse gene homologous to the human DAZ gene has been cloned and named Dazl (DAZ?like). Dazl is autosomal and expressed in the testis and also at a low level in the ovary. Male mice homozygous for the Dazl null allele have small testes with a few spermatogonia and almost complete absence of germ cells beyond the spermatogonial stage, suggesting the requirement of Dazl for entry or progression through meiosis. However, its exact cellular functions have not been understood yet. In order to investigate cellular functions of Dazl, we decided to isolate candidate interacting protein genes of the mouse Dazl, using yeast two?hybrid screening. A number of candidate Dazl?interacting proteins have been isolated, such as Bprp, Acf, Hgs, Murr1, Nbak3 and Ranbp9, but dynein light chain 1 (Dlc1) was most predominant. A strong interaction of Dazl with Dlc1 suggests that Dazl might function as an mRNA adaptor to the dynein motor complex.

키워드

DAZ; Dazl; male infertility; yeast two-hybrid screening; Dlc1

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