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Characterization of programmed cell death in the silkworm thoracic ganglia during postembryonic periods

Animal Cells and Systems 2007년 11권 1호 p.23 ~ 31
김순옥, 김미영, 송화영, 김진희, 강필돈, 이봉희,
소속 상세정보
김순옥 ( Kim Soon-Ok ) - Korea University School of Life Sciences and Biotechnology
김미영 ( Kim Mi-Young ) - Korea University School of Life Sciences and Biotechnology
송화영 ( Song Hwa-Young ) - Korea University School of Life Sciences and Biotechnology
김진희 ( Kim Jin-Hee ) - Korea University School of Life Sciences and Biotechnology
강필돈 ( Kang Pil-Don ) - National Institute of Agricultural Science and Technology Department of Agricultural Biology
이봉희 ( Lee Bong-Hee ) - Korea University School of Life Sciences and Biotechnology

Abstract


Programmed cell death was characterized in the silkworm thoracic ganglia TG1, TG2 and TG3 during postembryonic periods by TUN EL assay. Apoptotic cells were detected in the three TGs of all larval stages except for day?1, 2 1stinstar larvae, in which no apoptotic cells were found. From day?7 5th larva, the numbers of apoptotic cells were dramatically increased and peaked on day?1 pupa and day?2 pupa and then abruptly decreased. Apoptotic cells finally disappeared in day?1 adult. In?vivo injection of 20?hydroxyecdysone (20E) into day?8 5th larva resulted in a striking decrease of apoptotic cells. Actinomycin D (Act D) or cycloheximide (CHX), injected into hemolymph of day?8 5th larva, resulted in a decrease of apoptotic cells in the three TGs. Injection of caspase?8 and ?3 inhibitors also blocked cellular apoptosis. These results will provide valuable information for understanding of cellular changes in the three TGs during metamorphosis of the insect species.

키워드

Cell apoptosis; thoracic ganglia; silkworm; 20E; TUNEL assay

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