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Changes in sex steroid hormones and ovarian development during artificial maturation of female eel, Anguilla japonica

Animal Cells and Systems 2007년 11권 2호 p.117 ~ 124
김대정, 배준영, 김응오,
소속 상세정보
김대정 ( Kim Dae-Jung ) - NFRDI Aquaculture Research Team
배준영 ( Bae Jun-Young ) - NFRDI Aquaculture Research Team
김응오 ( Kim Eung-Oh ) - National Fisheries Research & Development Institute Inland Aquaculture Research Institute

Abstract


The present study demonstrates the changes in body weight (BW) and plasma sex steroid hormone profiles during artificial maturation induced by human chorionic gonadotropin (HCG) or salmon pituitary extract (SPE) injections in cultured eel, Anguilla japonica, kept in seawater for 3 months. In the weekly SPE?injected female group, BW was relatively stable during vitellogenesis. Following induction of vitellogenesis, females exhibited a rapid increase of BW, and the oocytes were observed to be in the migratory nucleus stage at the end of the experiment. Plasma testosterone (T) and estradiol?17β (E2) levels increased slightly during vitellogenesis and peaked at an average of 5.82 ng/mL and 4.76 ng/mL, respectively, at the end of the experiment. In the weekly control and HCG?injected female groups, BW slowly decreased during the experimental period, and the oocytes of the two groups were observed to be at the primary yolk globule stage. In the weekly HCG?injected female group, plasma T and E2 levels increased slightly during vitellogenesis and decreased afterward. In the control female group, however, plasma T and E2 levels were not altered during the experimental period. Furthermore, plasma 17α,20β?dihydroxy?4?pregnen?3?one (DHP) was not detected in all experimental groups. Fertility and hatching rates of SPE?injected females were significantly higher in those that ovulated 15 h after DHP injection than 18 h. These results indicate that long rearing in seawater increases responsiveness to SPE in ovarian maturation of the Japanese eel, resulting in shortened period from completion of vitellogenesis by sex steroid hormone production.

키워드

female eel; Anguilla japonica; sex steroid hormone; ovulation; fertilization; seawater rearing

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