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Interaction of microtubule-associated protein 1B light chain (MAP1B?LC1) and p53 represses transcriptional activity of p53

Animal Cells and Systems 2008년 12권 2호 p.69 ~ 75
김정웅, 이소연, 정미희, 장상민, 송기현, 김철홍, 김유진, 최경희,
소속 상세정보
김정웅 ( Kim Jung-Woong ) - Chung-Ang University College of Natural Science Department of Life Science
이소연 ( Lee So-Youn ) - Chung-Ang University College of Natural Science Department of Life Science
정미희 ( Jeong Mi-Hee ) - Chung-Ang University College of Natural Science Department of Life Science
장상민 ( Jang Sang-Min ) - Chung-Ang University College of Natural Science Department of Life Science
송기현 ( Song Ki-Hyun ) - Chung-Ang University College of Natural Science Department of Life Science
김철홍 ( Kim Chul-Hong ) - Chung-Ang University College of Natural Science Department of Life Science
김유진 ( Kim You-Jin ) - Chung-Ang University College of Natural Science Department of Life Science
최경희 ( Choi Kyung-Hee ) - Chung-Ang University College of Natural Science Department of Life Science

Abstract


The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses, and can trigger apoptosis in many cell types including neurons. In this study, we have shown that Microtubule?associated protein 1B (MAP1B) light chain interacts with tumor suppressor p53. MAP1B is one of the major cytoskeletal proteins in the developing nervous system and essential in forming axons during elongation. We also demonstrate that both p53 and MAP1B?LC1 interact in the nucleus in HEK 293 cells. Indeed, we show that the MAP1B?LC1 negatively regulates p53?dependent transcriptional activity of a reporter containing the p21 promoter. Consequently, MAP1B light chain binds with p53 and their interaction leads to the inhibition of doxorubicin?induced apoptosis in HEK 293 cells. Furthermore, these examinations might be taken into consideration when knock?down of MAP1B?LC1 is used as a cancer therapeutic strategy to enhance p53’s apoptotic activity in chemotherapy.

키워드

MAP1B-LC1; p53; transcriptional activity; apoptosis; protein interaction

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