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Changes in cytochrome c oxidase and NO in rat lung mitochondria following iron overload

Animal Cells and Systems 2009년 13권 2호 p.105 ~ 112
김미선, 홍민아, 송은숙,
소속 상세정보
김미선 ( Kim Mi-Sun ) - Sookmyung Women’s University College of Natural Sciences Division of Life Science
홍민아 ( Hong Min-A ) - Sookmyung Women’s University College of Natural Sciences Division of Life Science
송은숙 ( Song Eun-Sook ) - Sookmyung Women’s University College of Natural Sciences Division of Life Science

Abstract


In this study, the effects of iron on cytochrome c oxidase (CcO) in rat lung mitochondria were examined. Similar to liver mitochondria, iron accumulated considerably in lung mitochondria (more than 2?fold). Likewise, the reactive oxygen species and nitric oxide (NO) content of mitochondria were increased by more than 50% and 100%, respectively. NO might be produced by nitric oxide synthase (NOS), eNOS and iNOS type, with particular contribution by NOS in mitochondria. The respiratory control ratio of ironoverloaded lung mitochondria dropped to nearly 50% due to increased state 4. Likewise, cytochrome c oxidase activity was lowered significantly to approximately 50% due to excess iron. Real?time PCR revealed that the expression of isoforms 1 and 2 of subunit IV of CcO was enhanced greatly under excess iron conditions. Taken together, these results show that oxidative phosphorylation within lung mitochondria may be influenced by iron overload through changes in cytochrome c oxidase and NO.

키워드

lung mitochondria; iron overload; cytochrome c oxidase subunit IV; nitric oxide; nitric oxide synthase

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