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Gene expression and regulation of wax moth transferrin by PAMPs and heavy metals

Animal Cells and Systems 2009년 13권 3호 p.297 ~ 304
한직현, 이지숙, 이창석, 고상균, 서숙재, 윤치영,
소속 상세정보
한직현 ( Han Jik-Hyon ) - Korea University School of Life Sciences and Biotechnology
이지숙 ( Lee Ji-Sook ) - Daejeon University Department of Biology
이창석 ( Lee Chang-Seok ) - Korea University School of Life Sciences and Biotechnology
고상균 ( Koh Sang-Kyun ) - Daejeon University Department of Biology
서숙재 ( Seo Sook-Jae ) - Gyeongsang National University Division of Life Science
윤치영 ( Yun Chi-Young ) - Daejeon University Department of Biology

Abstract


A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G. mellonella transferrin had only one iron?binding site at its N?terminal region. Semi?qRT PCR was conducted to investigate tissue?specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up?regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen?associated molecular patterns (PAMPs) including LPS, lipoteichoic acid (LTA), glucan, and even chitin.

키워드

transferrin; wax moth; cDNA cloning; pathogenassociated molecular pattern; PAMP; iron; cadmium

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