잠시만 기다려 주세요. 로딩중입니다.

Amplified fragment length polymorphism analysis and genetic variation of the pinewood nematode Bursaphelenchus xylophilus in South Korea

Animal Cells and Systems 2010년 14권 1호 p.31 ~ 36
정종우, 한혜림, 류성희, 김원,
소속 상세정보
정종우 ( Jung Jong-Woo ) - Seoul National University School of Biological Sciences
한혜림 ( Han Hye-Rim ) - Korea Forest Research Institute Division of Forest Insect Pests and Diseases
류성희 ( Ryu Sung-Hee ) - Seoul National University School of Biological Sciences
김원 ( Kim Won ) - Seoul National University School of Biological Sciences

Abstract


The pinewood nematode Bursaphelenchus xylophilus causes pine wilt disease and is a serious economic concern for the forest industry of South Korea. To achieve effective control with limited resources, it is necessary to clarify the transmission routes and mechanisms of dispersal of this organism. Highly polymorphic and easy-to-use molecular markers can be used for investigating this aspect. In this study, we evaluated the usefulness of amplified fragment length polymorphisms (AFLPs) for investigating the genetic variations of B. xylophilus and related individuals from China, Japan, and South Korea. The AFLP patterns obtained in our study were similar to the microsatellite patterns reported in a previous study; our AFLP patterns indicated high genetic variability and cryptic genetic structure, but did not indicate any peculiar geographic structure. Moreover, the genetic distances between individuals suggested that the Korean population was affected to a greater extent by the Chinese population than the Japanese population. Further, the gene flow among the related species appeared to be limited; however, there may be also the possibility of genetic introgression among species. These results confirm the usefulness of AFLPs for understanding the epidemiology of pine wilt disease, thereby contributing to the effective control of this disease.

키워드

AFLPs; pinewood nematode; Bursaphelenchus xylophilus; pine wilt disease

원문 및 링크아웃 정보

등재저널 정보