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Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression

Animal Cells and Systems 2010년 14권 4호 p.275 ~ 282
정우상, 조진구, 인경민, 김종민, 조기호, 박정미, 문상관, 김경욱, 박성욱, 피재호, 박상규, 정윤화, 박헌용, 고창남,
소속 상세정보
정우상 ( Jung Woo-Sang ) - Kyung Hee University Kyung Hee Medical Center Hospital of Oriental Medicine Department of Cardiovascular and Neurologic Diseases
조진구 ( Cho Jin-Gu ) - Dankook University Department of Molecular Biology
인경민 ( In Kyung-Min ) - Dankook University Department of Molecular Biology
김종민 ( Kim Jong-Min ) - Dankook University Department of Molecular Biology
조기호 ( Cho Ki-Ho ) - Kyung Hee University Kyung Hee Medical Center Hospital of Oriental Medicine Department of Cardiovascular and Neurologic Diseases
박정미 ( Park Jung-Mi ) - Kyung Hee University East-West Neo Medical Center Department of Stroke and Neurological Disorders Center
문상관 ( Moon Sang-Kwan ) - Kyung Hee University Kyung Hee Medical Center Hospital of Oriental Medicine Department of Cardiovascular and Neurologic Diseases
김경욱 ( Kim Kyung-Wook ) - Kyung Hee University Kyung Hee Medical Center Hospital of Oriental Medicine Department of Cardiovascular and Neurologic Diseases
박성욱 ( Park Seong-Uk ) - Kyung Hee University East-West Neo Medical Center Department of Stroke and Neurological Disorders Center
피재호 ( Pyee Jae-Ho ) - Dankook University Department of Molecular Biology
박상규 ( Park Sang-Gyu ) - CHA University Department of Biomedical Science
정윤화 ( Jeong Yoon-Hwa ) - Dankook University Department of Food Science and Nutrition
박헌용 ( Park Heon-Yong ) - Dankook University Department of Molecular Biology
고창남 ( Ko Chang-Nam ) - Kyung Hee University East-West Neo Medical Center Department of Stroke and Neurological Disorders Center

Abstract


Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1 (VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1 leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from down-regulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1 and VCAM-1 gene expression.

키워드

chunghyul-dan; chemotaxis; cell adhesion; endothelial cells; transmigration

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