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Isolation and characterization of microsatellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis

Animal Cells and Systems 2011년 15권 1호 p.37 ~ 43
안동하, 박미현, 정재호, 오미진, 김상희, 정종우, 민기식,
소속 상세정보
안동하 ( Ahn Dong-Ha ) - Inha University Department of Biological Sciences
박미현 ( Park Mi-Hyun ) - Inha University Department of Biological Sciences
정재호 ( Jung Jae-Ho ) - Inha University Department of Biological Sciences
오미진 ( Oh Mi-Jin ) - Seoul National University Department of Biological Sciences
김상희 ( Kim Sang-Hee ) - Korea Polar Research Institute
정종우 ( Jung Jong-Woo ) - Ewha Womans University Department of Science Education
민기식 ( Min Gi-Sik ) - Inha University Department of Biological Sciences

Abstract


The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight microsatellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These microsatellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

키워드

microsatellite loci; freshwater crayfish; Cambaroides similis; Korea; population genetics

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