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Toxicoproteomic identification of TiO2 nanoparticle-induced protein expression changes in mouse brain

Animal Cells and Systems 2011년 15권 2호 p.107 ~ 114
전유미, 박슬기, 이미영,
소속 상세정보
전유미 ( Jeon Yu-Mi ) - Soonchunhyang University Department of Medical Biotechnology
박슬기 ( Park Seul-Ki ) - Soonchunhyang University Department of Medical Biotechnology
이미영 ( Lee Mi-Young ) - Soonchunhyang University Department of Medical Biotechnology

Abstract


A proteomic analysis of the proteins in mouse brain that were differentially expressed in response to TiO2 nanoparticles was conducted to better understand the molecular mechanism of TiO2 nanoparticle-induced brain toxicity at the protein level. A total of 990 proteins from mouse brain were resolved by two-dimensional gel electrophoresis. A comparative proteomic analysis revealed that the expression levels of 11 proteins were changed by more than 2-fold in response to TiO2 nanoparticles: eight proteins were upregulated and three were downregulated by TiO2 nanoparticles. In addition, the activities of several antioxidative enzymes and acetylcholine esterase were reduced in TiO2 nanoparticle-exposed mouse brain. The protein profile alterations seem to be due to an indirect effect of TiO2 nanoparticles, because TiO2 nanoparticles were not detected in the brain in this investigation.

키워드

proteomics; TiO2 nanoparticles; mouse; brain

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