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New polymorphic microsatellite markers in the Korean mi-iuy croaker, Miichthys miiuy, and their application to the genetic characterization of wild and farmed populations

Animal Cells and Systems 2012년 16권 1호 p.41 ~ 49
안혜석, 김은미, 이장욱, 김대정, 김이청,
소속 상세정보
안혜석 ( An Hye-Suck ) - National Fisheries Research and Development Institute New Strategy Research Center
김은미 ( Kim Eun-Mi ) - National Fisheries Research and Development Institute New Strategy Research Center
이장욱 ( Lee Jang-Wook ) - National Fisheries Research and Development Institute New Strategy Research Center
김대정 ( Kim Dae-Jung ) - National Fisheries Research and Development Institute New Strategy Research Center
김이청 ( Kim Yi-Cheong ) - National Fisheries Research and Development Institute New Strategy Research Center

Abstract


Eighteen new polymorphic microsatellite markers were developed for the Korean mi-iuy croaker (Miichthys miiuy, Perciformes, Sciaenidae), and allelic variability was compared between a wild population in Mokpo, Korea, and a hatchery population in Tongyeong, Korea. All loci were amplified readily and demonstrated allelic variability, with the number of alleles ranging from 5 to 37 in the wild population, and from 4 to 12 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, to be 0.74 and 0.78 in the hatchery population samples, and 0.79 and 0.86 in the wild samples. These results indicate lower genetic variability in the hatchery population compared with the wild population, and significant genetic differentiation between the wild population and the hatchery samples (F ST=0.058, P < 0.001). These microsatellite loci may be valuable for future population genetic studies, monitoring changes in the genetic variation within stocks in a commercial breeding program, conservation genetics, and molecular assisted selective breeding of the mi-iuy croaker in the future.

키워드

Korean mi-iuy croaker; Miichthys miiuy; microsatellite loci; genetic marker; genetic variability

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