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Identification of histone methyltransferase RE-IIBP target genes in leukemia cell line

Animal Cells and Systems 2012년 16권 4호 p.289 ~ 294
손혜주, 김지영, 이상명, 서상범,
소속 상세정보
손혜주 ( Son Hye-Ju ) - Chung-Ang University College of Natural Science Department of Life Science
김지영 ( Kim Ji-Young ) - Chung-Ang University College of Natural Science Department of Life Science
이상명 ( Rhee Sang-Myung ) - Chung-Ang University College of Natural Science Department of Life Science
서상범 ( Seo Sang-Beom ) - Chung-Ang University College of Natural Science Department of Life Science

Abstract


Histone methylation has diverse functions including transcriptional regulation via its lysine or arginine residue methylation. Studies indicate that deregulation of histone methylation is linked to human cancers including leukemia. Histone H3K27 methyltrnasferase response element II binding protein (RE-IIBP), as a transcriptional repressor to target gene IL-5, interacts with HDAC and is over-expressed in leukemia patient samples. In this study, we have identified that hematopoiesis-related genes GATA1 and HOXA9 are down-regulated by RE-IIBP in K562 and 293T cells. Transient reporter analysis revealed that GATA1 transcription was repressed by RE-IIBP. On the other hand, HOXA9 and PBX-related homeobox gene MEIS1 was up-regulated by RE-IIBP. These results suggest that RE-IIBP might have a role in hematopoiesis or leukemogenesis by regulating the transcription of target genes, possibly via its H3K27 methyltransferase activity.

키워드

RE-IIBP; histone methyltransferase; transcription; GATA1; MEIS1

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