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Administration of rhIL-2 upregulates HGF in the cirrhotic liver of partial hepatectomized rats

Animal Cells and Systems 2013년 17권 3호 p.179 ~ 185
전미애, 권현정, 김용현, 한국일, 남궁우, 백영준, 이승희, 김완종, 한만덕,
소속 상세정보
전미애 ( Jeon Mi-Ae ) - Soonchunhyang University College of Natural Sciences Department of Biology
권현정 ( Kwon Hyun-Jung ) - Soonchunhyang University College of Natural Sciences Department of Biology
김용현 ( Kim Yong-Hyun ) - Soonchunhyang University College of Natural Sciences Department of Biology
한국일 ( Han Kook-Il ) - Soonchunhyang University College of Natural Sciences Department of Biology
남궁우 ( Nam Kung-Woo ) - Soonchunhyang University College of Natural Sciences Department of Biology
백영준 ( Baik Yeong-Jun ) - BMI Korea Company Research Laboratory
이승희 ( Lee Sung-Hee ) - BMI Korea Company Research Laboratory
김완종 ( Kim Wan-Jong ) - Soonchunhyang University College of Natural Sciences Department of Biology
한만덕 ( Han Man-Deuk ) - Soonchunhyang University College of Natural Sciences Department of Biology

Abstract


Liver regeneration is a process involving highly organized and ordered tissue growth triggered by the loss of liver tissue. This process has remained a fascinating topic in medicine. In this study, we examined the effects of recombinant human interleukin-2 (rhIL-2) in rats under cirrhotic conditions. Thirty rats were divided into three groups, and liver cirrhosis was induced by injecting diethylnitrosamine for 2 weeks and CCl4 for 8 weeks. After induction of cirrhosis, rhIL-2 was injected daily into their tail vein for three days and then hepatocyte growth factor (HGF), immunocytes in the blood, and TNF-α production were analyzed. The analysis showed that TNF-α was significantly downregulated in the liver without significant changes in nitric oxide and caspase-3/7 activities in primary cultured liver cells. In terms of the number of immunocytes, there were no significant differences between the control and the treated groups. However, HGF levels were significantly higher in the treated groups than the control (saline administration) group. Treatment of rhIL-2 significantly increased the proliferation of primary cultured liver cells. The proliferation rates of primary cultured liver cells were correlated with the upregulation of HGF by treatment with rhIL-2 in vivo and in vitro. These results suggest that rhIL-2 affects liver regeneration, which is at least related to the upregulation of HGF under cirrhotic conditions in rats.

키워드

hepatocytes; recombinant human interleukin-2; TNF-a; caspase-3; HGF

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