Effects of various photoperiods on Kisspeptin and reproductive hormones in the goldfish, Carassius auratus
신현숙, 송진아, 최지용, 김나나, 최영재, 성시내, 박미선, 민병화, 최철영,
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신현숙 ( Shin Hyun-Suk ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
송진아 ( Song Jin-Ah ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
최지용 ( Choi Ji-Yong ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
김나나 ( Kim Na-Na ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
최영재 ( Choi Young-Jae ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
성시내 ( Sung Si-Nae ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
박미선 ( Park Mi-Seon ) - National Fisheries Research and Development Institute Aquaculture Management Division
민병화 ( Min Byung-Hwa ) - National Fisheries Research and Development Institute Aquaculture Management Division
최철영 ( Choi Cheol-Young ) - Korea Maritime and Ocean University Division of Marine Environment and Bioscience
Abstract
The study aimed to test differences in the hormones of the hypothalamus?pituitary?gonad axis, Kisspeptin (Kiss) and sex steroids, and the sexual maturation of goldfish (Carassius auratus) according to various photoperiods: 10 h light (L):14 h dark (D) (short day), 12L:12D (control), and 14L:10D (long day). To investigate the sexual maturation under various photoperiods, quantitative real-time PCR (QPCR) assays for salmon gonadotropin-releasing hormone, chicken gonadotropin-releasing hormone, gonadotropin hormones (GTHs), Kisspeptin 1 (Kiss1), and its receptor, G protein-coupled receptor 54 (GPR54), were developed. mRNA expression was monitored in cultured pituitary cells (in vitro) of various photoperiod groups. Furthermore, we injected or treated the animals with Kiss and found that Kiss treatment increases the mRNA expression levels of GTHs. We measured the plasma follicle-stimulating hormone (FSH), luteinizing hormone (LH), and 17α-hydroxypregnenolone levels. The gonadotropin-releasing hormones (GnRHs), GTHs, Kiss1/GPR54 mRNA expression, plasma FSH, LH, and 17α-hydroxypregnenolone levels in the 14L:10D group were significantly higher than in the other groups. These results suggest that light length regulates sex maturation by GnRH and Kiss1 in the brain of the goldfish.
키워드
gonadotropin-releasing hormone; kisspeptin; photoperiod; sex maturation; sex steroid hormone
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