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Embryoid bodies formation from chicken primordial germ cells

Animal Cells and Systems 2015년 19권 3호 p.168 ~ 174
Xiong Hui, Pu Yabin, Hu Qingyun, Shan Zhiqiang, Hu Pengfei, Guan Weijun, Ma Yuehui,
소속 상세정보
 ( Xiong Hui ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Pu Yabin ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Hu Qingyun ) - University of Jiamusi College of Basic Medicine
 ( Shan Zhiqiang ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Hu Pengfei ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Guan Weijun ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Ma Yuehui ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences

Abstract


Primordial germ cells (PGCs) were demonstrated to be multipotential because of their differentiation ability in all embryonic lineages and because the pluripotential nature of PGCs caters for recent researches on stem cells. PGCs were cultured in suspension to form embryoid bodies (EBs). The characterization of PGCs and EBs was assessed by immunofluorescence technique, reverse transcription-polymerase chain reaction (RT-PCR) and paraffin section for Haematoxylin and Eosin (HE) stain. We established a stable chicken PGC line in vitro and prepared masses of EBs from PGCs. We also demonstrated that PGCs expressed stage-specific and stem cell-specific surface makers: SSEA-1, SSEA-3, Oct4, and Sox2. EBs expressed specific genes from three germ layers: gene AFP from entoderm, gene GATA6 from mesoderm, and gene Sox3 from ectoderm. The HE staining illustrated that EBs developed different cell types; relatively larger EBs (440 μm in diameter) were obtained, which contract rhythmically. We concluded that chicken PGCs were suitable for the formation of EBs. Abundant larger size EBs could be prepared in an effective way with our protocol, which could construct a good animal model and provide a useful clinical platform in studying human embryonic development and cell transplantation field.

키워드

embryoid bodies; primordial germ cells; differentiation

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