A novel chitinase from the earthworm, Eisenia andrei
김대환, 김진세, 박인용, 곽희진, 이동호, 조성진, 박순철,
소속 상세정보
김대환 ( Kim Dae-Hwan ) - Chung-Ang University Department of Life Science
김진세 ( Kim Jin-Se ) - Chungbuk National University Department of Biology
박인용 ( Park In-Yong ) - Chungbuk National University Department of Biology
곽희진 ( Kwak Hee-Jin ) - Chungbuk National University Department of Biology
이동호 ( Lee Dong-Ho ) - Chung-Ang University Department of Life Science
조성진 ( Cho Sung-Jin ) - Chungbuk National University Department of Biology
박순철 ( Park Soon-Cheol ) - Chung-Ang University Department of Life Science
Abstract
A novel chitinase gene, EaChi, and its expression pattern from the earthworm, E. andrei are demonstrated. Based on a deduced amino acid sequence, in EaChi, two specific domains for GH family 18 are well conserved with two essential amino acid residues for enzyme activity. The phylogenetic analysis shows that earthworm chitinase, EaChi, is evolutionarily close to other lophotrochozoan chitinases. The expression pattern analysis of EaChi indicates that the major expression is localized at intestinal epithelium and epidermis, possibly suggesting that the prime functions of the chitinase activity could be related to not only digestive process but also self-defending immunity as a biochemical barrier to protect the invasion of chitin-containing pathogens, including fungi, nematodes and protozoa.
키워드
Earthworm; novel chitinase; phylogenetic tree; expression pattern; self-defense
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